University of Georgia Research Foundation, Inc.

Yuheng Lin

Yajun Yan

5-hydroxytryptophan (5-HTP), a precursor of serotonin, is produced in a microbial host cell. A modified bacterial phenylalanine 4-hydroxylase (P4H) catalyzes the tryptophan 5-hydroxylation reaction. Optionally the host cell includes a cofactor regeneration mechanism, allowing continuous production of 5-HTP without supplementation of exogenous cofactors.

Microbial approach for the production of 5-hydroxytryptophan

Provided herein are methods for producing an ortho-hydroxylated phenylpropanoid. In one embodiment the method includes culturing a microbe that includes HpaBC activity in the presence of a phenylpropanoid substrate. Also provided are genetically engineered microbes engineered to have greater levels of HpaB and/or HpaC than a control microbe.

Methods for hydroxylating phenylpropanoids

The invention provides a recombinant microorganism that has been genetically engineered to contain metabolic pathway for the production of muconic acid from a salicylic acid intermediate. The genetically engineered metabolic pathway comprises both biosynthetic and biodegradative elements.

Microbial production of muconic acid and salicylic acid

Provided herein are methods for the biosynthesis of 4-hydroxycoumarin. In one embodiment, provided herein are genetically engineered microbes that include a metabolic pathway for the production of 4-hydroxycoumarin. Also provided are methods for using the genetically engineered microbes to produce 4-hydroxycoumarin, and using the 4-hydroxycoumarin as the starting point for the synthesis of other compounds.

Genetically engineered microbes and methods for producing 4-hydroxycoumarin

Microorganisms are genetically engineered to synthesize caffeic acid from simple carbon sources via a tyrosine intermediate by means of a dual pathway that utilizes both endogenous and engineered enzymatic activities.

Growing community of inventors

Bogart, GA, United States of America

Yuheng Lin