Other

Tianjin University of Science and Technology

Henan Julong Biological Engineering Co., Ltd

Yuhang Zhou

Ning Chen

Qingyang Xu

Xiaoguang Fan

Jing Li

Xiaodong Liu

Bochao Liu

Shuai Liu

Yongchao Liu

Jiajia Teng

Mengtao Zhang

Chaochao Sun

Yuanqing Ji

Jun Yang

Bo Wang

FuSheng Pan

Hao Xu

Cheng Kun Li

YiFei Xu

Jiang Liu

Yuyu Dou

Zuzheng Wu

Hongyu Zhu

YuAn Chen

Yu Bi

Huajie Cao

Jun Yi

Pei Xie

Junyu Tian

Yuanyong Gong

The present invention belongs to the bioengineering field, and relates to a method for fermentation production of L-theanine by using angenetically engineered bacterium. The engineered bacterium is obtained by serving a strain as an original strain, wherein the strain is obtained after performing a single copy of T7RNAP, a dual copy of gmas, xylR knockout, and sucCD knockout on anW3110 genome, and by integrating genes xfp, pta, acs, gltA, and ppc, and knocking out ackA on the genome. The present invention has a high yield, and stable production performance; after 20-25 h, L-theanine has a titer of 75-80 g/L, and the yield is up to 52-55%. The fermentation broth is purified by membrane separation in combination with a cation-anion resin series technique. Moreover, the one-step crystallization yield is 72.3% and the L-theanine final product has a purity of 99%.

Method for producing L-theanine via fermentation by a genetically engineered bacterium and the application thereof

The disclosure discloses a genetically engineered strain for sarcosine production as well as a construction method and application. The genetically engineered strain is obtained by usingas a host and by integrating a single copy of imine reductase gene dpkA on its genome; singly copying citrate synthase gene gltA; knocking out glyoxylate cycle inhibitor gene iclR; knocking out malate synthase gene aceB; integrating a single copy of isocitrate lyase gene aceA; integrating a single copy of membrane-bound transhydrogenase gene pntAB; knocking out 2-ketate reductase gene ycdW; integrating a single copy of phosphoenolpyruvate carboxylase gene ppc; and knocking out pyruvate kinase gene pykF. After system metabolism transformation, the engineered strain can synthesize sarcosine with glucose and methylamine as main raw materials. The sarcosine titer can reach 10 g/L after fermentation for 30 h in a 5 L fermenter.

Genetically engineered bacterium for sarcosine production as well as construction method and application

Genetically engineered bacterium ofand method for fermentation production of L-theanine thereof

A tracked vehicle is provided. The tracked vehicle includes a frame, a driving mechanism installed in the frame, and two track wheels respectively arranged on the left and right sides of the frame. The driving mechanism drives the track wheels to rotate. The track wheel includes a track body, a first main support, a second main support and a third main support provided in the track body. The first main support, the second main support and the third main support are connected to the vehicle frame through the first, second and third connecting rods respectively. The second main support is provided with an auxiliary support frame having one end hinged to the second main support, and the other end of the auxiliary support frame is provided with a first roller contacting with the inner side of the track body.

Tracked vehicle

Growing community of inventors

Tianjin, China

Yuhang Zhou