Nanjing Tech University

Yuhan Yang

Yong Chen

Pingkai Ouyang

Dong Liu

Hanjie Ying

Di Zhang

Qingguo Liu

Ming Lei

Huanqing Niu

Bin Yu

Shishi Wu

The invention discloses a recombinantfor producing lysine by biofilm continuous fermentation and a construction method thereof, wherein the recombinantis constructed by overexpressing a protease gene FtsH in a; and the construction method comprises the following steps of: (1) performing PCR on a genome of the, and amplifying the FtsH gene to obtain an amplified FtsH gene segment; (2) cloning the FtsH gene segment to an overexpression plasmid to obtain a recombinant plasmid; and (3) introducing the recombinant plasmid into the, and screening to obtain the recombinant. The invention has the beneficial effects that thefor overexpressing the protease FtsH is constructed in the invention, and a film-forming ability of theis enhanced, such that a yield of continuous immobilized fermentation of theis increased by 38.2% than that of free fermentation of an original bacterium, and a fermentation cycle is shortened by 26.4%.

Recombinant corynebacterium glutamicum for producing lysine by biofilm continuous fermentation and construction method thereof

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Jiangsu, China

Yuhan Yang