Life Technologies Corporation

Yufang Wang

Barnett B Rosenblum

Michael Previte

Theo Nikiforov

Peter B Vander Horn

Cheng-Yao Chen

Guobin Luo

Alexander J Mastroianni

Jeremy Gray

Marc Glazer

Marian Peris

Hongye Sun

Christina E Inman

Michael Phelan

Zhaohui Zhou

Ronald L Cicero

Jamshid Temirov

Stefanie Yukiko Nishimura

Hongyi Wang

Wolfgang Hinz

James A Ball

Dmitriy Gremyachinskiy

Prasanna Krishnan Thwar

David Light

Phil Waggoner

Paul M Kenney

Roman Rozhkov

Joseph Koscinski

Mohammad Alanjary

Paul Kenney

Wofgang Hinz

A sensor component includes a sensor including a sensor surface and a reaction site in cooperation with the sensor and exposing the sensor surface. The reaction site including a reaction site surface. A surface agent is bound to the reaction site surface or the sensor surface. The surface agent includes a surface active functional group reactive with Bronsted base or Lewis acid functionality on the reaction site surface or the sensor surface and including distal functionality that does not have a donor electron pair.

Surface treatment of semiconductor sensors

Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

Nucleotide transient binding for sequencing methods

A method of forming a polymer matrix array includes treating a surface within a well of a well array with a surface compound including a surface reactive functional group and a radical-forming distal group; applying an aqueous solution including polymer precursors to the well of the well array; and activating the radical-forming distal group of the surface coupling compound with an initiator and atom transfer radical polymerization (ATRP) catalyst to initiate radical polymerization of the polymer precursors within the well of the well array to form the polymer matrix array.

Matrix arrays and methods for making same

A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

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San Carlos, CA, United States of America

Yufang Wang