Jecho Laboratories Inc.

The United States of America, As Represented by the Secretary, Department of Health and Human Services

Shanghai Jiao Tong University

Jecho Biopharmaceuticals Co Ltd.

Yueqing Xie

Jianwei Zhu

Hua Jiang

Huili Lu

Lei Zhang

Lei Han

Junsheng Chen

George Mitra

Samir Hussein Shaban

Trevor Lane Broadt

Cedric Cagliero

Manyu Luo

Kai Ding

Baohong Zhang

Andrew Burnette

Jianwei Sean Zhu

The present disclosure discloses a method for expressing and preparing a bivalent bispecific antibody. In the present disclosure, each portion of a bivalent bispecific antibody and an immune hybrid protein thereof is respectively expressed in a suitable prokaryotic or eukaryotic cell system, separated and purified by high-performance affinity chromatography, and then spliced in vitro by trans-splicing reaction mediated by an intein, to prepare the bivalent specific antibody and an immune hybrid protein thereof.

Bivalent bispecific antibody hybrid protein expression and preparation methods

Methods for preparing a recombinant protein from a bacterium are provided. The method includes constructing an expression vector including two promoters. Each of the two promoters attaches a secretion signal peptide to one polypeptide of a protein. The protein attached with the two promoters and secretion signal peptides is then cloned into the expression vector to provide a recombinant expression plasmid. The recombinant expression plasmid is transformed into a host cell. A fermentation process is performed to grow the host cell and to induce an expression to synthesize polypeptides in the host cell and to transport the polypeptides to an outside of a cytoplasm of the host cell, such that the polypeptides are released in a soluble form in a growth medium of the host cell. The polypeptides are assembled into a three-dimensional structure of the protein. The protein is captured from the growth medium.

Method for preparing recombinant protein from bacterium and composition containing the same

Described are improved processes for production and purification of nucleic acid-containing compositions, such as non-naturally occurring viruses, for example, recombinant polioviruses that can be employed as oncolytic agents. Some of the described improved processes relate to improved processes for producing viral DNA template. Also described are improved processes for chromatography purification of nucleic acid-containing compositions, in which the nucleic acid is quantified in chromatography fractions using a rapid detection method of the one or more nucleic acid sequences of the nucleic acid-containing composition, such as detection by real time RT-qPCR. In addition, improved processes for production and purification of oncolytic poliovirus, such as PVS-RIPO, are described. Compositions generated using these methods are also provided.

Processes for production and purification of nucleic acid-containing compositions

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Frederick, MD, United States of America

Yueqing Xie