Medical University of South Carolina

Battelle Memorial Institute

Yuehuei H An

Anna Gutowska

Vladimir A Mironov

Franklin A Young, Jr

A gelling cell culture medium useful for forming a three dimensional matrix for cell culture in vitro is prepared by copolymerizing an acrylamide derivative with a hydrophilic comonomer to form a reversible (preferably thermally reversible) gelling linear random copolymer in the form of a plurality of linear chains having a plurality of molecular weights greater than or equal to a minimum gelling molecular weight cutoff, mixing the copolymer with an aqueous solvent to form a reversible gelling solution and adding a cell culture medium to the gelling solution to form the gelling cell culture medium. Cells such as chondrocytes or hepatocytes are added to the culture medium to form a seeded culture medium, and temperature of the medium is raised to gel the seeded culture medium and form a three dimensional matrix containing the cells. After propagating the cells in the matrix, the cells may be recovered by lowering the temperature to dissolve the matrix and centrifuging.

Reversible gelling culture media for in-vitro cell culture in

The invention provides an adjustable ligament anchor for attaching a ligament to a bone. The ligament anchor comprises a housing having an exterior surface, an interior surface, an intra-articular end and an opposite extra-articular end. The interior surface defines a bore that extends longitudinally through the housing, joining the ends. A member is dimensioned to be received within the bore and having a first end and an opposite second end, wherein the ligament is attached to the first end. Adjusting means comprise a longitudinal chamber opening through the second end, and extending into at least a portion of the member. The chamber has threads and is dimensioned to receive a screw having a shaft with threads complimentary to threads in chamber.

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Charleston, SC, United States of America

Yuehuei H An