East China University of Science and Technology

Yuanxing Zhang

Yue Ma

Ping Zhang

Xiaolong Wang

Xiangshan Zhou

Jinjia Wang

Peng Bai

Dongling Zhao

Provided is a method of eliminating the dependence of methanol induced promoter on a single methanol carbon source for expressing foreign polypeptide. The method comprises activating the expression of the promoter requiring methanol induction by increasing the expression quantity of Mit1 polypeptide in cells of methylotrophic yeast, so that the promoter originally depending on methanol induction no longer depends on single methanol and can also express foreign polypeptide.

Method of eliminating dependence of methanol induced promoter on single methanol carbon source

A novel polyvalent attenuated live bacterial vaccine for preventing and curing vibriosis of cultivated fish is provided. The vaccine mainly comprises attenuated deletion strain ofwithout marker gene, which has significant low toxicity, but remains immunogenicity against wild type strain of, as compared with wild type strain MVM425. Moreover, the vaccine strain has excellent cross immunoprotection against. The attenuated live vaccine made from the vaccine strain is effective to prevent and cure vibriosis of fish resulted from wild type strain ofand

Polyvalent attenuated live vaccine for preventing and curing vibriosis of cultivated fish

Growing community of inventors

Shanghai, China

Yuanxing Zhang