Restoragen, Inc.

Medtronic, Inc.

Nps Pharmaceuticals, Inc.

Bionebraska, Inc.

Other

Yuannan Xia

Peng Luan

Fred W Wagner

Daniel J Strydom

Jin Seog Seo

James Arthur Williams

Barton Holmquist

Scott Harley

Mary J Bossard

Edwin H Merrifield

H Edward Grotjan

Julie Heinrich

The invention provides an inclusion body fusion partner to increase peptide and polypeptide production in a cell.

Methods of DNA constructs for high yield production of polypeptides

Methods and DNA constructs for high yield production of polypeptides

The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence.

Method for enzymatic production of GLP-2(1-33) and GLP-2(1-34) peptides

The invention provides methods and nucleic acid constructs to express clostripain. The source of the coding region for recombinantly expressed clostripain is

Methods and constructs for high yield expression of clostripain

Method for universal enzymatic production of bioactive peptides

The present invention provides novel promoter sequences obtained from Chlorella virus. The invention includes gene constructs comprising a promoter sequence of the invention operably linked to a DNA sequence encoding a structural gene. The invention also provides vectors and host cells for expressing product encoded by the structural gene of a gene construct of the invention amd cells transformed with the heterologous gene operably linked to the promoter.

Plant containing a gene construct comprising a chlorella virus promoter and a lac operator

Chlorella virus promoters

A method and recombinant assay cell for detecting growth hormone releasing hormone (GHRH) in a sample, the method includes exposing a recombinant cell to the sample and measuring transcription of a reporter gene. A suitable recombinant cell includes a reporter gene operatively connected to a cAMP-responsive promoter and a GHRH-responsive protein whose binding to GHRH induces the production of cAMP. GHRH present in an assay sample results in the GHRH-responsive protein activating the production of cAMP, which then activates the c-AMP-responsive promoter to express the reporter protein. The amount of reporter protein produced is quantitatively correlated to the amount of GHRH in the sample. In one embodiment, the protein is a cell surface receptor for GHRH.

Bioassay for growth hormone releasing hormone

The present invention provides novel promoter sequences obtained from Chlorella virus. The invention includes gene constructs comprising a promoter sequence of the invention operably linked to a DNA sequence encoding a structural gene. The invention also provides vectors and host cells for expressing a product encoded by the structural gene of a gene construct of the invention and cells transformed with the heterologous gene operably linked to the promoter.

Growing community of inventors

Lincoln, NE, United States of America

Yuannan Xia