Jiangnan University

Yuanfa Liu

Jinwei Li

Chen Cao

Zhaojun Zheng

Jiang Jiang

Yongjiang Xu

Yinghong Zhai

Peirang Cao

Qi Zhang

Li Zhou

Guiyang Shi

Xingguo Wang

Liting Zhao

Zhongyang Ding

Zhenghua Gu

Ying Wang

Mengmeng Xu

Liang Zhang

Jiaxin Li

Yin Chen

Zhanyang Guo

Dewei Sun

Qingling Wang

Kangyi Zhang

Xiangyun Qian

Zhihua Song

Junge Song

Binbin Nian

Youling Xiong

Shucheng Zhang

Yunzhi Jiang

The present disclosure discloses a method for modification of mung bean protein and preparation of simulated egg pulp based on the modified protein. A commercial mung bean protein isolate is used as a raw material in the present disclosure, then subjected to pH shift modification, ultrasonic treatment and protein-glutaminase treatment for compound modification to prepare a high-functionality modified mung bean protein isolate; and the modified mung bean protein isolate is used as a raw material, using high-speed emulsification and high-pressure homogenization are conducted to obtain a high-stability mung bean protein-based simulated egg pulp. In the present disclosure, the mung bean protein isolate prepared by compound modification has good solubility, emulsifying property and gelling property. The mung bean protein-based simulated egg pulp is in the form of a stable emulsion and has good fluidity and moderate gelling property, and uniformity and stability can still be maintained for a long time.

Method for modification of mung bean protein and preparation of simulated egg pulp based on the modified protein

The disclosure discloses a method for processing oil crops with, and belongs to the technical field of fermentation. The method includes the step of inoculating the(such asand) that can produce carotenoid into a fermentation medium that contains oil-rich oil crops for solid state fermentation to obtain oil and oil crop meal rich in carotenoid. The carotenoid as a fermentative metabolite of thehas bioactivities of resisting oxidation, preventing vascular sclerosis, enhancing immunity and preventing cancers. Contents of carotenoid in the oil and oil crop meal acquired by the method can be up to 9.071 μg/g and 8.062 μg/g correspondingly. By the method, the oil and oil crop meal rich in carotenoid can be acquired at the same time by just once fermentation and once oil pressing without additional functional substances, and thus the production cost of the functional oil and the fermentation oil crop meal is greatly reduced.

Method for processing oil crops with rhodotorula

The disclosure discloses a method for preparing functional grease through segmented solid-state fermentation of mixed fungi, and belongs to the field of edible oil processing. According to the disclosure, soybeans are used as a main fermentation substrate, for the requirements for different functional nutrition, the soybeans are matched with other oil seeds, fungus segmented solid-state fermentation is used and combined with a supercritical extraction fractionation technology, so that the purposes of improving the aftertaste of soybean oils and generating different flavors are achieved, and the nutritional value of the grease is improved. The fungus segmented solid-state fermentation of the disclosure can improve the oil yield, improve the fatty acid composition of the grease, and increase the content of main flavor substances such as pyrazines, alcohols and phenols, and the sensory functions such as flavor and color of the grease more meet the requirements of consumers for innovative products. Fermented dregs can be further processed to produce byproducts like functional polypeptides, so that sustainable green development is realized.

Method for preparing functional grease through segmented solid-state fermentation of mixed fungi

The disclosure discloses a method for quickly and accurately analyzing polyphenol content in rapeseed oil, and belongs to the field of analysis of natural compounds. The separation method of the disclosure uses acetonitrile-water as an extractant to extract polyphenols from the rapeseed oil, and cooperates with a Cadsorbent for purification, and then performs separation and purification. Compared with the traditional liquid-liquid extraction and solid-phase extraction, the method has an average recovery rate of polyphenols in the rapeseed oil of 81.31% to 102.95%, and RSDs of 0.86% to 8.03%, and has higher accuracy and precision. The method of the disclosure not only uses less organic reagents and causes less environmental pollution, but also reduces matrix interference and improves purification efficiency through optimization of the adsorbent. The method of the disclosure not only is simple to operate and low in cost, but also has less matrix interference and accurate results, and is suitable for the qualitative and quantitative determination of polyphenols in the rapeseed oil.

Method for quickly and accurately analyzing polyphenol content in rapeseed oil

The disclosure discloses a method for preparing a lipoamino acid compound based on deep eutectic solvents, and belongs to the field of organic synthesis. The preparation method of the disclosure uses a fatty acid and an amino acid as substrates, uses a deep eutectic solvent as a medium, and directly and efficiently prepares a single-chain lipoamino acid surfactant under the catalysis of lipase CALB. Alternatively, Amano lipase M is used as a catalyst to efficiently catalyze the reaction of an amino acid containing a plurality of amino groups with a fatty acid to prepare a Gemini amino acid. The method of the disclosure does not need to undergo protection and deprotection of the amino acid, and does not involve commonly used fatty acid chlorides. Compared with the traditional chemical synthesis methods, the disclosure has the advantages of greenness, safety, high selectivity, high yield, etc., and has very important potential application value.

Method for preparing lipoamino acids based on deep eutectic solvents

The disclosure provides an active peptide with an anti-lipid oxidation function and a preparation method and application thereof and belongs to the technical field of plant-derived biologically active peptides. In the disclosure, oil processing by-products, namely oil crops after oil extraction, are used as the raw materials, and the raw materials are subjected to the steps of protein extraction, infrared pretreatment, proteolysis, freeze-drying, lipophilic part extraction, vacuum concentration and drying to prepare an anti-lipid oxidation peptide having the functional characteristics of scavenging DPPH free radicals, chelating metal ions, inhibiting lipid peroxidation, prolonging vegetable oil oxidation induction time, improving emulsion stability and the like. The anti-lipid oxidation peptide can be used as a natural antioxidant in the storage and preservation of lipid and other lipid-containing food, the problems of product deterioration and harmful product production caused by lipid oxidation are solved, and the shelf life of food is prolonged.

Preparation method and application thereof of peptides with anti-lipid-oxidation capability

The disclosure discloses a production method of marbled beef, and belongs to the field of food processing. The processing steps of the method are: freezing, thawing of beef, preparation of a gel emulsion, injection, wrap shaping, quick freezing, cutting and packaging, and hot dipping. The preparation method of the process of the disclosure can realize high-efficiency and standardized industrial production. By injecting beef with the formulated gel emulsion, the fat content and high-quality protein content of the beef can be increased, thereby improving the sensory properties and flavor of the beef, and producing high-quality beef. The gel emulsion for preparing marbled beef of the disclosure is liquid and is low in viscosity when the temperature is higher than 40&#xb0; C., and therefore the gel emulsion is convenient for injection into beef, and can be uniformly dispersed in beef to form a delicate marble pattern. When the temperature is lower than 20&#xb0; C., the gel emulsion forms a solid in beef tissue. The beef prepared according to the method of the disclosure shows a white fat pattern at low temperature (0-25&#xb0; C.), and is closer to natural marbled beef in appearance and structure.

Approach for processing marble-like beef

The invention discloses a method for preparing rapeseed oil by a semi-solid aqueous enzymatic process, belonging to the field of functional foods and health care products. The present invention first prepares a semi-solid rapeseed paste with 3.5-4.5% moisture content, which is hydrolyzed by a mixture of pectinase, cellulase and alkaline protease to extract rapeseed oil. The resulting rapeseed oil contains high levels of active ingredients including totaxin, sterol, phenols and beta-carotene. The rapeseed oil of the invention can be added into animal feeds, which helps to reduce animal blood lipid levels and body weight. It can significantly reduce the contents of total triglyceride, total cholesterol and LDC-C in the blood, and, at the same time, increases the level of HDL-C in the blood. In addition, the rapeseed oil prepared by the present invention can relieve hepatic steatosis in hyperlipemia rats.

Method for preparing rapeseed oil by semi-solid aqueous enzymatic treatment

The present invention provides a method for oil extraction from dehydrated, which is related to the field of food biotechnology. The method combines a low temperature and low oxygen heat pump dehydration system with a microwave-assisted frozen-blasting dehydration system fordehydration, resulting in formation of a large number of micro porous structures in dehydratedthat is good for subsequent oil extraction. The dehydration and extraction process can be separated in the present invention. The low temperature and low oxygen heat pump dehydration system may be installed in shrimp boats to dehydratebefore transportation so as to increase the effective payloads of shrimp boats. The extraction process of subcritical fluid assisted with ultrasonic operation is carried out under low temperature and low oxygen conditions, therefore the oxidation of active ingredients is avoided to a large extent. Compared with existing methods, the present invention not only possess excellent dehydration efficiency but also uses less time and energy.

Method for extracting oil from dehydrated

The invention provides a method for producing high-quality lard or beef tallow with low cholesterol content. The method of the invention uses enzyme hydrolysis, centrifugation separation and homogenization-assisted embedding technique to obtain high-quality lard or beef tallow with low cholesterol levels. The method uses aqueous enzymatic extraction (AEE) techniques to separate lard or beef tallow from the raw material of pork or beef fat, and uses the homogenization-assisted embedding technique to remove cholesterol from the lard and beef tallow products. The method of the invention produces high quality lard with good oxidative stability at a high yield and can meet the Chinese national standard for first grade lard. The lard of the invention needs no further degumming process, eliminating the tedious refinery steps.

Method for producing high quality animal oil with low cholesterol levels

Disclosed is a method for preparing L-&#x3b1;-Glycerylphosphorylcholine with high yields and purity. The method uses phospholipase A-based enzymatic hydrolysis, ion-exchange resin purification and silica gel column chromatography to prepare L-&#x3b1;-glycerylphosphorylcholin with purity up to 99.8% and a final yield up to 78.4%. The method disclosed is simple, cost-effective, environmentally friendly, and adaptable to industrial applications.

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Wuxi, China

Yuanfa Liu