10X Genomics, Inc.

Integenx, Inc.

Yuan Li

Michael Schnall-Levin

Christopher Hindson

Paul Hardenbol

Andrew D Price

Timothy M Woudenberg

William D Nielsen

David Eberhart

Ezra Van Gelder

Stephen J Williams

James Ogg

Dean S Burgi

Kevin D Ness

Benjamin J Hindson

Serge Saxonov

Donald Masquelier

Mirna Jarosz

Landon Merrill

This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.

Sample preparation, processing and analysis systems

This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

Compositions and methods for sample processing

Methods and systems for processing polynucleotides

Method and systems for processing polynucleotides

Growing community of inventors

Dublin, CA, United States of America

Yuan Li