Mgi Tech Co., Ltd.

Johnson Electric S.a.

Other

Tencent Technology (shenzhen) Company Limited

Bgi Shenzhen

Zte Corporation

Yuan Jiang

Hui Fu Jiang

Wenwei Zhang

Radoje Drmanac

Xia Zhao

Chunyu Geng

Qiaoling Li

Kai Tian

Huaiqian Xu

Bo Wang

Rui Feng Qin

Li Chen

Jing Guo

Cheng Shun Du

Andrei Alexeev

Xiaojun Ji

Yaqiao Li

Hongyan Han

Shengmao Liu

Yong Li

James Ching Sik Lau

Dong Li

Jing Zhao

Hui Li

Pengfei Zhang

Wei Pang

Lingyu He

Tong Wang

Ao Chen

Di Wang

Ji Yu Liang

Wei Cheng

Wen-wei Zhang

Rongrong Guo

Chao Dong

Ruoying Chen

Shujin Fu

Evan Hurowitz

Chu Li

Ziyu Yin

Hui Jiang

Liangzhen Du

Guanying Guo

Fanzi Wu

Xiaoshan Su

Guangying Guo

Jie Ruan

Hailong Sun

A separation and collection device for cells and biomolecules includes an annular tube and several magnetic beads configured to identify and bind with different cells or molecules in the biological sample. The annular tube defines a through hole for the biological sample to exit. Sizes of different groups of magnetic beads are different. An aperture size of a through hole is changeable such that the plurality of groups of magnetic beads can flow through the through hole in a certain order. A testing system including the separation and collection device is further disclosed.

Separation and collection device for cells and biomolecules, and testing system

This application discloses a method and apparatus for obtaining a virtual image, a computer device, a computer-readable storage medium, and a computer program product, belonging to the technical field of artificial intelligence. The method includes obtaining a target image generation model by fusing a first image generation model and a second image generation model, the first image generation model being trained based on a sample original image, the sample original image retaining object ontology features of a sample object, and the second image generation model being trained based on a sample virtual image having target attributes; and obtaining a target virtual image corresponding to an original image of a target object based on the target image generation model, the target virtual image retaining object ontology features of the target object and having the target attributes.

Method and apparatus for obtaining virtual image, computer device, computer-readable storage medium, and computer program product

Provided are an adaptor element in a bubble shape, a method of constructing a sequencing library with such an adapter element. The adaptor element is a hybrid formed with a long-chain nucleic acid A and a short-chain nucleic acid B. The hybrid is in the bubble shape with paired regions at two terminals and a non-paired region in the middle.

Bubble-shaped adaptor element and method of constructing sequencing library with bubble-shaped adaptor element

Provided herein are compositions, methods, and kits for enriching for one or more nucleic acid sequences of interest in a sample. The methods include providing a circular ligase, one or more 5' hook probes and/or one or more 3&#x2032; hook probes and contacting the sample comprising the nucleic acids with the circular ligase and one or more 5&#x2032; hook probes and/or one or more 3&#x2032; hook probes under conditions to allow the hook probes to selectively bind to the one or more nucleic acid sequences of interest, and under conditions to form one or more hook products, each hook product comprising the hook probes and the one or more nucleic acid sequences of interest.

Methods for hybridization based hook ligation

Provided are a vesicular adaptor and a single-chain cyclic library constructed by using the adaptor. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantage of high throughput sequencing, high accuracy and simple operations.

Vesicular adaptor and uses thereof in nucleic acid library construction and sequencing

The present invention provides a primer for nucleic acid random fragmentation and a nucleic acid random fragmentation method. The primer consists of a plurality of upstream random primers and downstream random primers. The sequence composition of the upstream random primers is 5'-X-Y-3&#x2032;, and the sequence composition of the downstream random primers is 5&#x2032;-P-Y&#x2032;-X&#x2032;-close-3&#x2032;, wherein Y and Y&#x2032; are random sequences, X is all or part of sequences of a sequencing platform 5&#x2032; end adaptor, X&#x2032; is all or part of sequences of a sequencing platform 3&#x2032; end adaptor, P is phosphorylation modification, and close is close modification. The primer of the present invention adopts double random anchoring of both the upstream random primers and the downstream random primers, and a DNA sample can be randomly broken.

Primer for nucleic acid random fragmentation and nucleic acid random fragmentation method

Provided are a vesicular linker and a single-chain cyclic library constructed by using the linker. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantages of high throughput sequencing, high accuracy and simple operations.

Vesicular linker and uses thereof in nucleic acid library construction and sequencing

Provided is a linker element and a method of using the linker element to construct a sequencing library, wherein the linker element consists of a linker A and a linker B, the linker A is obtained through the complementary pairing of a long nucleic acid strand and a short nucleic acid strand, the 5' end of the long strand has a phosphoric acid modification, and the 3&#x2032; end of the short strand has an enclosed modification, with enzyme sites in the short strand; and the linker B is a nucleic acid single strand, and the 3&#x2032; end thereof can be in a complementary pairing with the 5&#x2032; end of the long strand of the linker A. Using the linker element of the present invention for constructing a sequencing library ensures the linking directionality of the linkers while solving the problems of fragment interlinking, linker self-linking and low linking efficiency, and reducing the purification reaction between steps, shortening the linking time and reducing costs.

Linker element and method of using same to construct sequencing library

A method and reagent for constructing a nucleic acid double-linker single-strand cyclic library. The method comprises: breaking a nucleic acid into nucleic acid fragments; connecting a first linker sequence; producing by amplification a first product provided with the first linker sequence at either end, where a U nucleobase site is provided on primer sequences and a nicking enzyme recognition sequence is either provided or not provided on same, and a first affinity tag is provided on one of the primer sequences; using USER enzyme to cleave the first product; cyclizing the cleaved first product; treating the cyclization product with either a phosphatase or a nicking enzyme; using a solid-phase vector for combination with a cyclized molecule; performing a restrictive gap translation reaction; removing by digestion any portion that did not undergo the restrictive gap translation reaction; connecting a second linker sequence; producing by amplification a second product provided with the second linker sequence at either end; denaturing the second product, and cyclizing a single-strand nucleic acid molecule. The method allows an increase in the length of library insert fragments, a simplified library construction process, reduced library construction time, and reduced library construction costs.

Method and reagent for constructing nucleic acid double-linker single-strand cyclical library

Provided are a method for constructing a nucleic acid single-stranded cyclic library and the reagents used therein. By the combination of interruption via a transposase with a restricted nick translation reaction, the method realizes a simple and rapid nucleic acid single-stranded cyclic library construction.

Method for constructing nucleic acid single-stranded cyclic library and reagents thereof

Disclosed are a nucleic acid fragmentation method and a sequence combination. The method comprises the following steps: subjecting a denatured nucleic acid to annealing and an extension reaction by using a single-stranded 5'-end extension primer, wherein the single-stranded 5&#x2032;-end extension primer comprises a sequencing platform adaptor sequence of a 5&#x2032; end and a connected random sequence, and the random sequence is subjected to annealing on a random site of the denatured nucleic acid; and directionally connecting a double-stranded 3&#x2032;-end adaptor sequence to the 3&#x2032; end of the nucleic acid generated in the extension reaction, and carrying out denaturalization and purification to obtain a fragmented single-stranded nucleic acid with adaptor sequences on two ends.

Method and a sequence combination for producing nucleic acid fragments

Provided is a method of constructing a sequencing library using an adaptor element in a bubble shape. The bubble-shaped adaptor is ligated to a DNA fragment respectively at the 3'-terminal and the 5&#x2032;-terminal i.e., two bubble-shaped adaptors with same sequences are ligated in one step. The bubble-shaped adaptor-ligated product is then amplified with a primer complementary to the 3&#x2032;-terminal of the long-chain nucleic acid of the bubble-shaped adaptor, so as to replace the non-paired sequence in the short-chain nucleic acid.

Method of constructing sequencing library with bubble-shaped adaptor element

The present invention relates to a system for video communication comprising a display having a reflective surface facing to a local user and a video camera mounted on a supporting structure and aimed toward the display; wherein said display displays dark screen images periodically; said camera captures of the local user's image reflected off from the surface during the dark screen image period. The captured reflective images of the local user are used to form a video stream for a remote user to view.

System and method for face-to-face video communication

An electric motor has a housing, a rotor rotatably installed in the housing, n permanent magnets with the same polarity fixed at an inner surface of the housing. The rotor is a wound armature having a plurality of teeth extending in a radial direction. The housing includes n inner projecting parts, the permanent magnets and the inner projecting parts being alternately arranged in a circumferential direction about the rotor. The magnets generate 2n magnetic circuits via the housing and the rotor, n being an integer greater than one. The size of at least a portion of a first air gap formed between the permanent magnets and the rotor is greater than the size of at least a portion of a second air gap formed between the inner projecting parts and the rotor.

Electric motor

Growing community of inventors

Shenzhen, China

Yuan Jiang