Industrial Technology Research Institute

Yu-Yu Lin

Pei-Shin Jiang

Cheng-Tao Wu

Yu-Ching Chang

Chung-Cheng Liu

Shiu-Chieh Lan

Chien-An Chen

Jenn-Yeh Fann

Hung-Chi Chien

Chih-Lung Lin

Ya-Ru Huang

Shu-Chin Chou

Shinn-Horng Yeh

Su-Jan Lee

Tzu-Hui Wu

Chia-Chun Chen

Chien-Ming Hsu

Chung-Ya Liao

Methods of amplifying and determining a target nucleotide sequence are provided. The method of amplifying the target nucleotide sequence includes the following steps. A first adaptor and a second adaptor are linked to two ends of a double-stranded nucleic acid molecule with a target nucleotide sequence respectively to form a nucleic acid template, in which the first adaptor includes a Y-form adaptor or a hairpin adaptor and the second adaptor is a hairpin adaptor. Then, a PCR amplification cycle is performed on the nucleic acid template to obtain a PCR amplicon of the target nucleotide sequence.

Method of amplifying and determining target nucleotide sequence

One embodiment of the disclosure provides a kit for detecting a mutation and/or polymorphism of a specific region in a target nucleotide sequence, including: at least one first primer consisting of a first segment and a second segment, wherein the first segment is a complementary strand of a first sequence and the second segment is a second sequence, and the 3' end of the first segment connects to the 5&#x2032; end of the second segment; a second primer being a third sequence; at least one third primer consisting of a third segment and a fourth segment, wherein the third segment is a fourth sequence and the fourth segment is a complementary strand of a fifth sequence, and the 3&#x2032; end of the third segment connects to the 5&#x2032; end of the fourth segment; and a fourth primer being a complementary strand of a sixth sequence, wherein the specific region includes rs1799853, rs1057910, rs2108622, rs9923231 and rs9934438.

Kit for detecting a mutation and/or polymorphism of a specific region in a target nucleotide sequence

Disclosed are methods of identifying microRNA motifs or microRNA precursors for a target gene or a set of target genes. Also disclosed are related computer-readable media.

MicroRNA precursors

MicroRNA motifs

The present invention is related to a method for manufacturing lead frames and a lead frame material including an intermediate layer and a top layer. The intermediate layer is composed of a layer of nickel-cobalt alloy having 5 to 30 wt. % of cobalt and a thickness of 3 to 20 microinches and a layer of nickel or nickel alloy having a thickness of 10 to 80 microinches. The intermediate layer can inhibit the diffusion of the base metal to the surface of the leads. The top layer consisting of gold or gold alloy, which is composed of gold and at least one metal selected from the group consisting of palladium, silver, tin and copper and has at least 60 weight percent gold, has a thickness of 0.1 to 5 microinches.

Method for manufacturing lead frames and lead frame material for semiconductor device

Growing community of inventors

Yilan County, Taiwan

Yu-Yu Lin