Institute of Microelectronics

Chugai Seiyaku Kabushiki Kaisha

Nxp B.v.

Agency for Science, Technology and Research

Globalfoundries Singapore Pte. Ltd.

National University of Singapore

Pharmalogicals Research Pte. Ltd.

Ciea International Inc.

Other

Yu Jau Chen

Tit Meng Lim

Masami Suzuki

Quanbo Zou

Koichi Matsubara

Uppili Sridhar

Tie Yan

Chunshan Yin

Atsuhiko Kato

Shinta Kobayashi

Cheong Min Hong

Chew Kiat Heng

Emmanuel Selvanayagam Zachariah

Masayuki Tsuchiya

Miho Watanabe

Soh Yun Siah

Siow Lee Chwa

Tatsumi Yamazaki

Hisafumi Okabe

Kenji Yoshida

Huajun Liu

Yoke Leng Lim

Janak Singh

Yubo Miao

Yanxia Shao

Juliana Sim

Etsuko Fujii

Jae Gon Lee

Ranganathan Nagarajan

Pang Dow Foo

Vaidyanathan Kripesh

Bangun Indajang

Julien Reboud

Vincent Ho

Chirayarikathuveedu Sankarapillai Premachandran

Ser Choong Chong

Yan Ping Wang

Shin Yun Ng

Masaki Yamazaki

Chie Kato

Janice Hao Yuan Liaw

Kum Cheong Tang

Andre Yao-Kuang Chung

Hui Fang Cui

Fwu-Shan Sheu

Jianshan Ye

Linus Tzu-Hsiang Kao

Provided is a cancer stem cell mass from which cells incapable of forming cancer are substantially removed and which has a characteristic property of reproducing a layered structure of a cancer tissue; a process for producing the cancer stem cell mass; and use of the cancer stem cell mass. A human cancer tissue was repeatedly grown in a NOG mouse, separated cancer cells from the grown cancer tissue, and tested and compared various cancer cell culture processes. As a result, a cancer stem cell composition which is homogeneous and is substantially free of the coexistence of cells capable of forming cancer and cells incapable of forming cancer in a mixed state can be produced successively by employing an attached culture process using a serum-free stem cell culture medium rather than a generally employed floating culture process.

Cancer stem cell population and method for production thereof

An objective of the present invention is to provide non-human animal models of cancer pathology, which mimic the hierarchical organization, cancer progression process, or biological property of human cancer tissues, and uses thereof. To achieve the objective described above, first, the present inventors transplanted cells of NOG-established cancer lines into NOG mice and morphologically observed the resulting tissue organization. As a result, the non-human animal models were demonstrated to exhibit pathologies (the hierarchical organization, cancer progression process, or biological properties of the cancer cells) similar to that of human cancer. Specifically, the present inventors succeeded in preparing non-human animal models exhibiting pathologies more similar to a human cancer, and cell culture systems using NOG-established cancer cell lines where the in vitro cell morphology is more similar to that of human cancer.

Method for searching and screening for target of anti-cancer agent using non-human animal model having NOG established cancer cell line transplanted therein

An integrated circuit having a fingered capacitor with multiple metal fingers formed in inverted-trapezoid-shaped trenches in a multi-layer structure having a polish stop layer over an ultra-low-K dielectric layer over a low-K dielectric layer over a dielectric cap layer. The ultra-low-K dielectric layer reduces capacitance variations between the fingers, while the polish stop layer prevents metal height variations that would otherwise result from performing CMP directly on the ultra-low-K dielectric layer. The layered structure may include another low-K dielectric layer over the polish stop layer that provides a soft landing for the CMP. The polish stop layer may be removed after the CMP polishing and another ultra-low-K dielectric layer may be formed to encapsulate the tops of the metal fingers in the ultra-low-K dielectric material.

Fingered capacitor with low-k and ultra-low-k dielectric layers

The purpose of the present invention is to provide: a cancer stem cell mass from which cells incapable of forming cancer are substantially removed and which has a characteristic property of reproducing a layered structure of a cancer tissue; a process for producing the cancer stem cell mass; and use of the cancer stem cell mass. For achieving the purpose, the present inventors grew a human cancer tissue repeatedly in a NOG mouse, separated cancer cells from the grown cancer tissue, and made a comparison of various cancer cell culture processes with each other. As a result, a cancer stem cell composition which is homogeneous and is substantially free of the coexistence of cells capable of forming cancer and cells incapable of forming cancer in a mixed state can be produced successively by employing an attached culture process using a serum-free stem cell culture medium rather than a generally employed floating culture process, and consequently the present invention has been accomplished.

Fingered capacitor with low-K and ultra-low-K dielectric layers

An integrated circuit has a first gated diode with one or more diode fingers. Each diode finger has an elevated gate, an underlying p-type diffusion, and an underlying n-type diffusion. Each diffusion has a base region and an annular side region located between the base region and the elevated gate such that the diffusions have increased lateral surface areas that support greater current levels for the diode finger, which enables gated diodes to be implemented with fewer fingers and therefore less layout area than equivalent conventional gated diodes that do not have elevated gates. The first gated diode can be implemented with an analogous second gated diode to form ESD-protection circuitry for the integrated circuit.

Gated diode having fingers with elevated gates

Fabrication of a slim split gate cell and the resulting device are disclosed. Embodiments include forming a first gate on a substrate, the first gate having an upper surface and a hard-mask covering the upper surface, forming an interpoly isolation layer on side surfaces of the first gate and the hard-mask, forming a second gate on one side of the first gate, with an uppermost point of the second gate below the upper surface of the first gate, removing the hard-mask, forming spacers on exposed vertical surfaces, and forming a salicide on exposed surfaces of the first and second gates.

Method of forming split-gate cell for non-volative memory devices

According to various embodiments, a microfluidic system for detecting a biological entity in a sample volume is provided. The microfluidic system may include: a chamber configured to receive the sample volume, wherein the chamber includes a detection region for detecting the biological entity; a first port in fluid communication with the chamber; and a second port including a filter in fluid communication with the chamber; and wherein a fluid provided to the first port or the second port flows between the first port and the second port through the chamber.

Microfluidic system for detecting a biological entity in a sample

An objective of the present invention is to facilitate the acquisition of antibody-producing cells that are infiltrating virus-infected cells, cancer cells, abnormal cells forming a benign hyperplasia, and the like, and to improve the efficiency of the production of antibodies as well as nucleic acids encoding them from the antibody-producing cells. The present inventors discovered that, when cancer tissues comprising infiltrating lymphocytes are transplanted into highly immunodeficient animals that do not have T cells, B cells, and NK cells and further exhibit a low IFN production ability, the differentiation and proliferation of infiltrating lymphocytes are unexpectedly promoted, and the number of plasma cells that produce antibodies recognizing cancer tissues increases dramatically, plasma cells can be separated easily, and antibodies or nucleic acids encoding them can be easily prepared from the plasma cells.

Methods of generating and using antibody-producing cells

Disposable units in current use for performing PCR are limited by their heat block ramping rates and by the thermal diffusion delay time through the plastic wall as well as by the sample itself. This limitation has been overcome by forming a disposable plastic chip using a simple deformation process wherein one or more plastic sheets are caused, through hydrostatic pressure, to conform to the surface of a suitable mold. After a given disposable chip has been filled with liquid samples, it is brought into close contact with an array of heating blocks that seals each sample within its own chamber, allowing each sample to then be heat treated as desired.

Shallow multi-well plastic chip for thermal multiplexing

Method of producing a nucleic acid encoding an antibody

A method for forming a semiconductor device is presented. The method includes providing a substrate prepared with first and second regions with a first device layer. A second device layer including nanocrystals is also formed. A cover layer is provided over the second device layer. The cover layer is patterned to expose portions of the second device layer in the first and second regions. The exposed portions of the second device layer in the first and second regions are processed to form modified portions. The processing of the exposed portions at least reduces the nanocrystals to a diameter below a threshold diameter in the modified portions. The modified portions are removed.

Patterning nanocrystal layers

A sensor array integrated electrochemical chip is provided wherein the chip has an array of electrodes. The array may be formed on a base plate bonded to a cover plate having an opening. The opening can be a window or a depression. The plates are bounded such that they define a cavity, with the array being within the cavity. Conducting lines for connecting the electrodes to electrochemical instruments may be formed on the same surface of the base plate on which the electrodes are formed. At least one of the electrodes may be covered by a coating doped with a ferrocene compound. The coating may be a supported bilayer lipid membrane doped with benzoylferrocene. The doped ferrocene compound may be oxidized.

Sensor array integrated electrochemical chip, method of forming same, and electrode coating

Growing community of inventors

Singapore, Singapore

Yu Jau Chen