Hitachi, Ltd.

Hitachi-high-technologies Corporation

Hitachi High-tech Corporation

Yu Ishige

Masao Kamahori

Tetsuyoshi Ono

Atsushi Kishioka

Masafumi Miyake

Yusuke Goto

Kuniaki Nagamine

Kotaro Yamashita

Yasuhisa Shibata

Hiroshi Sasaki

Kunio Harada

Sakuichiro Adachi

Satoshi Ozawa

Yoshinobu Kohara

Hideyuki Akiyama

Shintaro Kubo

Haruyoshi Yamamoto

Takahiro Ando

Wolfgang Schuhmann

Stefan Klink

A sample separation device to introduce a sample to be separated to an inside of the sample separation device, centrifuge the sample, and collect a desired liquid after separation, includes: a sample introducing portion introducing the sample into the sample separation device; a liquid surface defining portion connected to the sample introducing portion; a first separating portion connected to the liquid surface defining portion; a second separating portion connected to the first separating portion; a third separating portion connected to the second separating portion; and a liquid extracting portion including the third separating portion and connected to the third separating portion, in which a tubular flow path is formed by at least the first separating portion and the second separating portion, in which an opening of the sample introducing portion and an opening of the liquid extracting portion are oriented in the same direction.

Sample separation device and sample separation method

An automated analyzer includes a plurality of measurement units including a measurement section which measures a sample, a suction container connected to the plurality of measurement units through a first path, a vacuum exhaust system connected to the suction container through a second path, a discharge section connected to the suction container through a third path, a pressure adjustment mechanism disposed in the second path, and a control section which performs control such that the suction container is vacuum-exhausted by the vacuum exhaust system.

Automated analyzer and liquid discharge method for automated analyzer

There is provided an electrolyte measuring device that can decrease a liquid amount used for measurement, in which stable sealing can be provided in connecting passages of ion selective electrodes to each other with no gap, while maintaining high measurement accuracy of an existing ion selective electrode, and a residing sample liquid can be greatly decreased. In a flow-type ion selective electrode, a sealing member is used, which can be brought into intimate contact with a passage connecting unit to near a passage hole. A gap regulating member is provided to keep a gap between the electrodes constant and to prevent the sealing member from being excessively pressed. An electrode case has a structure suitable for the sealing member for allowing the alignment and holding of the sealing member.

Flow-type ion selective electrode, electrolyte concentration measuring device using the same, and biochemical automatic analyzer

There is provided a solid-contact ion-selective electrode having a high potential stability and potential reproducibility. The ion-selective electrode includes an ion sensitive film having a Group-1 element ion, a Group-2 element ion, a hydronium ion or an ammonium ion as a target ion, an ion occluding material layer, and a conductive electrode, wherein the ion occluding material contained in the ion occludes material layer occluding the target ion, the ion occluding material being a Prussian blue analog represented by the structure formula AM[M'(CN)]&#x25a1;.kHO. Herein, A is one type or a plurality of types of Group-1 elements, Group-2 elements, hydronium, or ammonium; M and M&#x2032; are one type or a plurality of types of transition metals; M or M&#x2032; includes at least one of nickel, cobalt, copper, silver, and cadmium; &#x25a1; is a vacancy in a porous coordination polymer; x and y are greater than zero; and a, z, and k are numbers equal to or greater than zero.

Ion-selective electrode, method of manufacture thereof, and cartridge

An electrolyte concentration measuring apparatus is provided with: a plurality of ion selective electrodes and one reference electrode; a sample introduction unit that introduces a sample solution to the plurality of ion selective electrodes and the reference electrode; a potential measuring unit that measures a voltage between the plurality of ion selective electrodes and the reference electrode; and a resistance measuring unit that measures a direct-current resistance of the plurality of ion selective electrodes.

Electrolyte concentration measuring apparatus and measuring method using same

To enhance the potential stability of a solid-electrode-type ion-selective electrode and reduce individual variations, the present invention is provided with an ion-sensitive membrane adapted to be in contact with a measurement solution, a mixture of graphite and liquid oil that is in contact with the ion-sensitive membrane, and a conductor that is in contact with the mixture of graphite and liquid oil.

Ion-selective electrode

To prevent lowering of sensitivity of a flow cell based on total reflection of light at an outer face of a glass capillary at a joint part with a pipe, the flow cell includes, at joint parts with a pipeto introduce solution to a glass capillaryand with a pipe to discharge solution from the capillary, an inorganic material layerthat reflects measurement light to modify the outer face of the glass capillary as well as a reinforcement layerto modify the surface thereof.

Flow cell and liquid analyzer

Provided is a potentiometric sensor chip in which the positional relationship among a reference electrode, a measurement electrode, and a sample inlet which enables measurement from the start of a reaction is defined, and further provided is a method for detecting the start time of the reaction. A very small amount of sample is measured with high accuracy. The very small quantity of sample is measured by a rate assay. When a reference electrode () is disposed between a sample inlet () and a measurement electrode (), a sample solution arrives at the reference electrode () earlier than at the measurement electrode (), whereby the surface potential of the measurement electrode () can be measured simultaneously when the sample solution arrives at the measurement electrode () and dissolves a reagent and thereby a reaction starts. The arrival of the sample solution at the measurement electrode () can be sensed by detecting the discontinuous change of the voltage observed by a voltmeter () at this time.

Potentiometric-sensor chip, potentiometric assay, and assay kit

A potentiometric sensor with suppressed leak current on the surface of an electrode and improved for a dynamic range and a response speed, in which a redox compound is immobilized through insulative molecules on the surface of a gold electrode, and a current between a source and drain of an insulated gate field-effect transistor along with reaction between an oxidized substance or a reduced substance produced by the reaction of a measured substance in a sample solution injector for supplying the sample solution containing the measured substance and an enzyme and a redox compound on the surface of the gold electrode, is monitored on real time to measure the change of the surface potential.

Potentiometric sensor and analytical element

The present invention aims to simplify the structure and a fabrication method of an ion-selective electrode in an ion concentration measuring device that measures an anion, particularly a chloride ion, in a biological component. To this end, in a potential difference measuring unit, a quaternary ammonium salt derivative serving as a ligand for an anion is immobilized to the surface of a gold electrode by using as a linker an insulative molecule forming a self-assembled monolayer. The potential difference measuring unit measures an electromotive force generated with anion binding, as an interface potential change on the surface of the gold electrode. In order to reduce the influence of adsorption of impurities on the electrode surface, a high-molecular weight polymer is physically adsorbed on the gold electrode and thus used when a biological component is measured.

Anion concentration measuring device and anion concentration measuring element

A crown ether derivative that acts as cation capturing ligand and alkanethiol having a longer carbon chain than a linker are immobilized, coexisting together, on the surface of a gold electrode, by using as the linker an insulating molecule (e.g., alkanethiol) that forms self-assembled monolayers. Electromotive force produced in association with cation coordination is measured by a potentiometer through a change in interfacial potential on the surface of the gold electrode. Further, an insulated gate field effect transistor formed on the same substrate as the gold electrode is used as the potentiometer. Furthermore, a straight-chain polymer physically adsorbed on the gold electrode is used in order to reduce the influence of the adsorption of impurities on the surface of the electrode during biological sample measurement.

Ion concentration measuring device and ion concentration measuring element

Disclosed is a DNA analysis method and a DNA analyzer whose signal intensity is not lowered even when a material at a higher density is measured. There is supplied dATP, dTTP, dGTP, or dCTP from a dATP solution vessel, a dTTP solution vessel, a dGTP solution vessel, or a dCTP solution vessel, and this causes an extension reaction of a double-stranded DNA immobilized to a bead, to yield pyrophosphoric acid. The pyrophosphoric acid is converted into a redox compound by the actions of a reagent and an enzyme contained in a reaction buffer in a reaction buffer vessel. The redox compound causes a variation in surface potential of a measuring electrode bearing an electrochemically active material immobilized thereto through an insulating molecule, and this variation causes a variation in drain current of a field-effect transistor electrically connected to the measuring electrode. Thus, the extension reaction is detected.

DNA analysis method and DNA analyzer

It is an object of this invention to measure small amounts of a plurality of sample solutions at the same time. The small amounts of sample solutions are respectively placed on measuring electrodes, a medium is placed across the plurality of sample solutions, a liquid joint of a reference electrode is brought into contact with the medium, and a potential difference between each of the measuring electrodes and the reference electrode via the medium is measured.

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Tokyo, Japan

Yu Ishige