Taipei Medical University

Yu-Hui Tsai

Li-Hsuan Chiu

Shih-Ching Chen

Wen-Fu Lai

Chin-Chean Wong

Yu-Wei Wu

The invention discloses a method of accelerating osteogenic differentiation and a composition thereof. The method comprises a step of adding type II collagen into stem/progenitor cells or osteoblasts to accelerate the osteogenic differentiation of the added cells, and the composition comprises type II collagen, and stem/progenitor cells or osteoblasts. Type II collagen can accelerate osteogenesis of mesenchymal stem cells (MSCs) much faster than does type I collagen. The said composition is effective to facilitate bone repair upon introduction of the composition into various osseous defects.

Method of accelerating osteogenic differentiation and composition thereof

The present invention provides a method for restoring native chondrocyte phenotype and functions of, and/or increasing type II collagen as well as aggrecan mRNA expression levels and GAG accumulation level in dedifferentiated chondrocytes which have been subcultured and expanded in vitro, which comprising culturing the said dedifferentiated chondrocytes in vitro with a medium comprising type II collagen, or its biologically active peptide fragment(s) or analogs with or without growth factor(s), wherein the type II collagen or its biologically active peptide fragment(s) or analogs are effective to restore chondrocyte phenotype and functions of, and/or to increase type II collagen and aggrecan expression levels and GAG accumulation level in the said dedifferentiated chondrocytes.

Method to restore cartilaginous phenotype of chondrocytes after cultured and expanded in vitro

The invention develops a high-throughput screening method based on charcoal-sorbent peptide binding assay (CPBA) which does not need a solid phase and can simultaneously detect plural target biomolecules in a sample. The method of the invention can achieve high-throughput screening of biomolecules (such as antibodies and antigens) with a molecular weight of more than 10 KD.

Growing community of inventors

Banciao, Taiwan

Yu-Hui Tsai