Orchid Biosciences, Inc.

Molecular Tool, Inc.

Orchid Biocomputer, Inc.

Yu-Hui Rogers

Michael T Boyce-Jacino

Stephen P Anderson

Mitchell A Friedman

Philip Goelet

Steve Anderson

The present invention provides a method for determining the nucleotide sequence of a nucleic acid molecule using primer oligonucleotides arrayed on a solid support and doing primer extension. By providing sequence information from both oligonucleotide primers in which the 3&prime; terminal nucleotide of the oligonucleotide primer is hybridized to the nucleic acid molecule and oligonucleotide primers in which the 3&prime; terminal nucleotide is not hybridized to the nucleic acid molecule the invention is advantageous in that less primers are needed to fully sequence a nucleic acid molecule. Where the 3&prime; terminal nucleotide of an oligonucleotide in the array does not hybridize to the nucleic acid molecule, the present invention provides for either not permitting oligonucleotides hybridized to nucleic acid molecules to be extended by polymerase-mediated incorporation of a single chain terminator nucleotide residue onto the 3&prime; terminus of said hybridized oligonucleotide, or permitting the removal of any non-hybridized nucleotide residues from the 3&prime; terminus of said hybridized oligonucloetides, so as to form a truncated primer oligonucleotide whose 3&prime; terminus is hybridized to said nucleic acid molecule. The invention also provides kits that can be used to implement the methods of the invention.

Method for analyzing the nucleotide sequence of a polynucleotide by oligonucleotide extension on an array

The invention concerns an apparatus for printing arrays of molecules and methods for printing these arrays. More particularly, the invention relates to a gene pen apparatus that may be used for delivering arrays of oligonucleotides on solid supports from a multiplicity of reagent reservoirs through flow controlling means. The flow controlling means may for example, be either a pin valve means or a wicking means.

Gene pen devices for array printing

Methods for the covalent, specific and reversible immobilization of nucleic acid molecules onto solid-phases by means of a reversible disulfide bond for nucleic acid molecule array preparation are described. These methods can be used to prepare reusable nucleic acid molecule arrays with hilgh specificity and high efficiency.

Covalent attachment of nucleic acid molecules onto solid-phases via

Methods for the covalent, specific and reversible immobilization of nucleic acid molecules onto solid-phases by means of a reversible disulfide bond for nucleic acid molecule array preparation are described. These methods can be used to prepare reusable nucleic acid molecule arrays with high specificity and high efficiency.

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Damascus, MD, United States of America

Yu-Hui Rogers