Ventana Medical Systems, Inc.

Yu-Heng Cheng

Lisa A Jones

Setareh Duquette

Javier Andres Perez-Sepulveda

Chih-Ching Lin

Jessica A Wifall

Javier A Perez Sepulveda

Jessica Wifall

A real time assay monitoring system and method can be used to monitor reagent volume in assays for fluid replenishment control, monitor assays in real-time to obtain quality control information, monitor assays in real-time during development to detect saturation levels that can be used to shorten assay time, and provide assay results before the assay is complete, enabling reflex testing to begin automatically. The monitoring system can include a real time imaging system with a camera and lights to capture images of the assay. The captured images can then be used to monitor and control the quality of the staining process in an assay, provide early assay results, and/or to measure the on-site reagent volume within the assay.

System and method for real time assay monitoring

A real-time imaging method and system for monitoring an assay process performed on a biological sample is described. In some embodiments, the method and system can be used to measure and control a reagent volume confined to a space between a cover and a substrate, which is particularly useful for controlling for evaporation in a thin-film staining environment. In particular embodiments, the disclosed reagent volume sensing and replenishment method and system are resistant to system noises generated during operation, for example, system noise due to vibration, field of view blockage by dispenser systems, differences in reagent colors, and changing tissue colors.

System and method for real-time volume control

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Northville, MI, United States of America

Yu-Heng Cheng