Institut Pasteur

Institut National De La Sante Et De La Recherche Medicale

Institut Pasteur and Centre National De La Recherche Scientifique

Yu-Chun Lone

François Lemonnier

Claude Auriault

Anthony Pajot

Philippe Kourilsky

Jean-Pierre Abastado

Estelle Mottez

Armanda Casrouge

Veronique Pancre

Véronique Pancre

David Ojcius

The present invention relates to transgenic mice and isolated transgenic mouse cells, the mice and mouse cells comprising a disrupted H2 class I gene, a disrupted H2 class II gene, a functional HLA class I transgene, and a functional HLA class II transgene. In embodiments, the transgenic mouse or mouse cells are deficient for both H2 class I and class II molecules, wherein the transgenic mouse comprises a functional HLA class I transgene and a functional HLA class II transgene. In embodiments, the transgenic mouse or mouse cell has the genotype HLA-A2HLA-DR1&#x3b2;2m&#xb0;IA&#x3b2;&#xb0;. The invention also relates to methods of using a transgenic mouse of the invention.

Transgenic mice having a human major histocompatibility complex (MHC) phenotype, experimental uses and applications

Transgenic mice having a human major histocompatability complex (MHC) phenotype, experimental uses and applications

An altered MHC class I determinant comprises .alpha..sub.1, .alpha..sub.2, .alpha..sub.3, .beta..sub.2 -microglobulin (.beta..sub.2 m) polypeptide domains encoded by a mammalian MHC class I locus in which the .alpha..sub.3 domain is covalently linked to the .beta..sub.2 M domain. An altered MHC class II determinant comprises .alpha..sub.1, .alpha..sub.2, .beta..sub.1, and .beta..sub.2 polypeptide domains encoded by a mammalian MHC class II locus, in which the domains are covalently linked to form a polypeptide comprising the .beta..sub.2 -.alpha..sub.2 -.alpha..sub.1 -.beta..sub.1 domains in sequence. The altered MHC class I and class II determinants can be associated with an antigen to elicit an immune response. The invention can be used in the immunization or treatment of diseases such as AIDS, multiple sclerosis, lupus erythematosus, toxic shock or snake bite.

Altered major histocompatibility complex (MHC) determinant and methods

Growing community of inventors

Paris, France

Yu-Chun Lone