National Yang Ming Chiao Tung University

Yu-Chie Chen

Cheng-Tai Chen

Wei-Yu Chen

Hong-Yi Lin

Chih-Chi Liu

Chun-Yuen Lo

Ya-Shiuan Lin

The present invention relates to a metal oxide nano-composite magnetic material, fabrication method, and method for linkage, enrichment, and isolation of phosphorylated species. The metal oxide nano-composite magnetic material comprises the magnetic iron oxide nanoparticle, a silica layer immobilized onto the magnetic iron oxide nanoparticle and a metal oxide layer coated onto the silica layer. The magnetic iron oxide nanoparticles can be used for absorbing microwave radiation to accelerate the enrichment and linkage for phosphorylated species onto the metal oxide nano-composite magnetic material. Furthermore, the magnetic property of magnetic iron oxide nanoparticles leads to isolation of the metal oxide nano-composite magnetic material-target species conjugates by simply employing an external magnetic field. The specificity of the metal oxide on the surface of the metal oxide nano-composite magnetic material for phosphorylated species also leads to the linkage/enrichment of phosphorylated species with high selectivity.

Metal oxide nano-composite magnetic material, fabrication method, and method for linkage, enrichment, and isolation of phosphorylated species

The invention provides a simple, rapid and cost-effective metal oxide-assisted laser desorption/ionization mass spectrometry (MOALDI MS) without the addition of light-absorbing organic-matrix, comprising the use of (a) an inorganic metal oxide with light absorbing capability as an assisting material to render desorption/ionization of samples in laser desorption/ionization mass spectrometry and (b) a citric acid buffer as the proton source for enhancing the ionization efficiency for analytes. Metal oxide assisting materials is not only restricted to the uses of films. Metal oxide nanoparticles are also suitable to be used as the assisting materials. Low matrix background, stable surface feature, homogeneous sample deposition, and wide detectable mass range are the merits of MOALDI MS.

Metal oxide-assisted laser desorption/ionization mass spectrometry

Growing community of inventors

Hsinchu, Taiwan

Yu-Chie Chen