Tsinghua University

Chang Chun Petrochemical Co., Ltd.

Chang Chun Plastics Co., Ltd.

Dairen Chemical Corporation

Yu-Chen Hu

Nam Ngoc Pham

Guan-Yu Chen

Chi-Yuan Chen

June-Yen Chou

Hsing-Yun Wang

Wen-Hsin Lo

Hsing-Yu Tuan

Vincent Jianan Liu

Shih-Yeh Lin

A gene editing system ofincludes a, a first gene editing fragment and a second gene editing fragment. The first gene editing fragment successively includes a first homology arm and a screening gene. The second gene editing fragment is connected to a C-terminus of the first gene editing fragment and includes a second homology arm, a Cas9 expression cassette and a sgRNA cassette. The Cas9 expression cassette successively includes a Cas9 promoter, a Cas9 gene and three nuclear localization sequences. The sgRNA cassette successively includes a sgRNA promoter, a first ribozyme, a targeting sequence, a scaffold and a second ribozyme. The first gene editing fragment and the second gene editing fragment are constructed as a linear fragment for gene editing of a chromosome of the

Transformant for producing dodecanedioic acid and method for producing dodecanedioic acid

The present disclosure relates to a transformant for producing 2,5-furandicarboxylic acid. The transformant for producing 2,5-furandicarboxylic acid includes aand at least one exogenous gene. The exogenous gene is an HmfH gene or an HMFO gene, and the exogenous gene is integrated into the chromosome of the

Transformant for producing 2,5-furandicarboxylic acid and preparation method for 2,5-furandicarboxylic acid

A method of inducing autophagy in a cell is achieved by contacting the cell with graphene oxide (GO) in an amount effective to induce autophagy in the cell, wherein the cell expresses at least one of TLR-4 (Toll-like receptor 4) and TLR-9 (Toll-like receptor 9). Differences between autophagy triggered by GO and other conventional agonists such as rapamycin have been observed. GO may activate autophagy in some cells that may not be triggered by rapamycin. The cell reveals no apparent apoptosis after treatment of the graphene oxide. A method of activating a Toll-like receptor in a cell is also herein provided.

Method of inducing autophagy and activating toll-like receptor

A recombinant baculovirus is provided for preparing picornavirus virus-like particles (VLP), wherein Chitinase A (ChiA) and Cathepsin V (v-cath) genes of the recombinant baculovirus are functionally disrupted and the recombinant baculovirus includes a picornavirus capsid protein gene under control of a strong promoter, and includes a protease gene configured for encoding a protease for hydrolyzing the capsid protein under control of a weak promoter. The recombinant baculovirus of the present invention may adopt High Five or Sf-9 cells for manufacturing enterovirus virus-like particles with improved stability and higher yields in comparison with the conventional arts. A method for preparing virus like particles is also herein provided.

Method for preparing virus-like particle and recombinant baculovirus used therein

A baculovirus expression vector achieves dual functions of (1) subunit vaccine by displaying the influenza surface protein for humoral immune responses; and (2) DNA vaccine by expressing influenza surface protein for long-acting cellular immune response. A method for inducing immunogenicity in a host is also disclosed.

Baculovirus expression vector and method therewith for generating immunogenicity in a host

A method for sustained expression of an exogenous gene forms a circular episomal plasmid to solve the problem of transient expression of baculovirus in the transduced mammalian cells caused by the dilution and degradation when mammalian cells replicate and also prevents dysfunctional cell metabolism.

Growing community of inventors

Hsinchu, Taiwan

Yu-Chen Hu