San Heh Pharmaceutical Corporation

Vor, Inc.

Yu-Chen Chen

Weiwei Gao

TsungFeng Wu

Disclosed are methods, systems, and devices for detecting biological analytes in a sample. The disclosed technology can be used to obtain readings of analyte concentration in a sample by imaging scattered light from an angled narrow beam illuminator. A fluid sample containing one or more biological, organic, and inorganic analytes including proteins, viruses, bacteria, phages, toxins, proteins, peptides, DNA, RNA, hormones, chemicals, drugs, and isotopes can be transferred to a microfluidic device having one or more channels with dimensions to generate capillary action for sample transport. The geometry of the microfluidic device may include a reservoir and sensing area, wherein an immunometric reaction can take place for the narrow beam scanning. The test particle may be coated with a specific binding member that is used to bind the binding pair member on an analyte in a sample. Test particles form the binding and the particle/analyte conjugate may be scanned.

Dual-image based bioimaging devices and techniques

Disclosed are methods, systems, and devices for detecting biological analytes in a sample. The disclosed technology can be used to obtain readings of analyte concentration in a sample by imaging scattered light from an angled narrow beam illuminator. A fluid sample containing one or more biological, organic, and inorganic analytes including proteins, viruses, bacteria, phages, toxins, peptides, DNA, RNA, hormones, chemicals, drugs, and isotopes can be transferred to a microfluidic device having one or more channels with dimensions to generate capillary action for sample transport. The geometry of the microfluidic device may include a reservoir and sensing area, wherein an immunometric reaction can take place for the narrow beam scanning. The test particle may be coated with a specific binding member that is used to bind the binding pair member on an analyte in a sample. Test particles form the binding and the particle/analyte conjugate may be scanned.

The present invention provides a process for preparing the nanoparticles. The process comprises first forming a water-in-oil emulsion from chitosan lactate, amoxicillin, dioctyl sodium sulfosuccinate, glutaraldehyde or bis[sulfosuccinimidyl] suberate, and oil, and sonicating the mixture of to form nanoparticles comprising chitosan crosslinked by dioctyl sodium sulfosuccinate and glutaraldehyde or by dioctyl sodium sulfosuccinate and bis[sulfosuccinimidyl] suberate, wherein the nanoparticles have an average diameter of 100-600 nm and have amoxicillin entrapped by the crosslinked chitosan. The present invention is also directed to nanoparticles comprising crosslinked chitosan and amoxicillin, wherein amoxicillin is entrapped by the crosslinked chitosan. The nanoparticles have an average diameter of 100-600 nm, and the entrapped amoxicillin is at least 5% (w/w) of the total weight nanoparticles.

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San Diego, CA, United States of America

Yu-Chen Chen