Nikon Corporation

Institut National De La Sante Et De La Recherche Medicale

Japan Science and Technology Agency

Institut Pasteur

The University of Tokushima

Yousuke Takahama

Philippe Kourilsky

Yoshitaro Nakano

Takeshi Nitta

Gabriel Gachelin

Irina Apostolov

Statistical data of a fluorescence population is obtained easily with high accuracy. To achieve this, an evaluation apparatus of fluorescence population of the present application includes an input unit inputting a fluorescence observation image (If) of a fluorescence population and a transillumination observation image (It) having a same field of view as the fluorescence observation image, a first detecting unit (Mt) detecting, as a first area, an area in which a cellular image exists on the transillumination observation image being input, a setting unit (If') setting, as a reference area, an area corresponding to the first area on the fluorescence observation image being input, and an obtaining unit obtaining fluorescence intensity data of a fluorescence image from the reference area in the fluorescence observation image.

Evaluation apparatus of fluorescence population, evaluation method of fluorescence population and computer readable storage medium

The aim of the present invention is to provide a method of acquiring immunological tolerance to a foreign DNA or its expression product whereby the foreign DNA such as a vector carrying a foreign gene incorporated thereinto or its expression product can be recognized not as non-self but as self; a method of sustaining a gene therapeutic effect whereby a rejection to a foreign DNA such as a vector carrying a foreign gene incorporated thereinto or its expression product can be avoided; and a non-human animal which has acquired immunological tolerance to a foreign DNA such as a vector carrying a foreign gene incorporated thereinto or its expression product. Fetal immature T lymphocytes transferred with a foreign DNA, such as a foreign gene-incorporated viral vector, are introduced into thymus and said foreign DNA is expressed in the thymus organ. The methods of transferring said foreign DNA into a fetal immature T lymphocyte include, for example, co-cultivating the fetal immature T lymphocytes with viral vector-infected virus producer cells.

Method of acquiring immunological tolerance

The invention concerns a pharmaceutical composition comprising at least a PIM-activated NKT cell and the use of at least one PIM and/or a PIM-activated NKT cell for treating a disease for which a granulomatous type of immune response is desired.

Method for activating NKT cells using PIMs

Growing community of inventors

Tokushima, Japan

Yousuke Takahama