Ajinomoto Co., Ltd.

Yousuke Nishio

Yoshihiro Usuda

Shinichi Sugimoto

Hisashi Yasueda

Kazuhiko Matsui

Chie Koseki

Kazuteru Yamada

Kosuke Yokota

Youko Yamamoto

A target substance can be produced by culturing a bacterium having an ability to produce 2-ketoglutaric acid or a derivative thereof, and an ability to produce xylonic acid from xylose, which is imparted with xylonate dehydratase activity, 2-keto-3-deoxyxylonate dehydratase activity and 2-ketoglutaric semialdehyde dehydrogenase activity, or in which these activities are enhanced, in a medium containing xylose as a carbon source to produce and accumulate the target substance in the medium, and collecting the target substance from the medium.

Method for producing a target substance by fermentation

A bacterium is described which belongs to the Enterobacteriaceae family, and has an ability to produce an L-amino acid, such as L-glutamic acid, L-arginine and L-threonine. The bacterium is modified so that the activity of a protein encoded by ydcI gene is decreased, thereby producing and accumulating the L-amino acid selected from L-glutamic acid, L-arginine, and L-threonine in the culture medium or cells of the bacterium when cultured in a culture medium. Subsequently, the L-amino acid is collected from the culture medium or the bacterium.

Bacterium capable of producing L-amino acid and method for producing L-amino acid

The present invention provides novel polypeptides and polynucleotides useful as biotechnological tools, specifically identified in a coryneform bacteriumssp.and methods of producing substances in organisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.

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The present invention provides polypeptides and polynucleotides involved in amino acid biosynthesis inand methods of producing amino acids in microorganisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.

The present invention provides polypeptides and polynucleotides involved in C1 assimilation inand methods of producing amino acids in microorganisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.

The present invention provides polypeptides and polynucleotides involved in central intermediates metabolism inand methods of producing amino acids in microorganisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.

Polynucleotides encoding polypeptides involved in intermediates metabolism of central metabolic pathway in methylophilus methylotrophus

Polynucleotides encoding polypeptides involved in amino acid biosynthesis in methylophilus methylotrophus

The present invention provides polypeptides and polynucleotides involved in the stress response to environmental changes inand methods of producing amino acids in microorganisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.

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Kawasaki, Japan

Yousuke Nishio