Technicon Instruments Corporation

Young R Kim

Leonard Ornstein

Michael J Malin

John F Cremins

Louis D Sclafani

Grace E Martin

Robert J Kanter

Henry Cook Waters, Iii

Hudson R Ansley

A flow cytometry method for reproducibly detecting and counting a lymphocyte population of interest in a leukocyte suspension or whole blood sample in which the red cells are subsequently lysed. The suspension (or sample) is combined with a reagent comprising a primary antibody, either native, carrying an attached enzyme or biotin or other label, and a fixative reagent, in either order. Where the enzyme is not attached, an enzyme is coupled specifically to the primary antibody. The fixed suspension is reacted with a color-producing enzyme-cytochemical reagent. The suspension, now including stained and unstained fixed cells, is passed through a flow cytometer and the cells are characterized and counted on the basis of their light-scattering and light-absorbing properties.

Method for discriminating surface stained lymphocytes

A composition is disclosed of blood serum sample preparation for improved, more accurate and precise, electrooptical method for measuring erythrocyte volumes, individually and as an average.

Reagent mixture and composition for treating red blood cells to effect

This invention relates to a method for the rapid determination of a differential white blood cell count in a sample, which method comprises:

Reagent for the determination of a differential white blood cell count

Method for the determination of a differential white blood cell count

A rapid one step, one reagent method is disclosed for the isovolumetric sphering and fixing of whole blood erythrocytes.

One step method for sphering and fixing whole blood erythrocytes

A method is disclosed of blood serum sample preparation for improved, more accurate and precise, electro-optical method for measuring erythrocyte volumes, individually and as an average.

Method for treating red blood cells to effect sphering and reagent

Procedures are disclosed for obtaining white blood cell counts and platelet counts by light scattering measurements in which anticoagulated blood samples are treated such that the morphology of the white cells (leukocytes) and platelets remain intact and permit the discrimination of platelets from white cells and of most classes of white cells from one another and their counting without the need for staining.

Process for preparing a cell suspension from blood for discrimination of

Blood is preserved by combining samples with a preservative reagent consisting of an aqueous mixture of a mono-, di- or trisaccharide component and formaldehyde.

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Hartsdale, NY, United States of America

Young R Kim