Lg Chem, Ltd

Lg Life Sciences, Limited

Lucky Corporation Co., Ltd.

Young Mee Lee

Seung Joon Lee

Bum Joon Kim

In Soo Kim

Soon Jae Park

Jung Hee Kim

Hee Ju Kim

Soon Chang Kwon

Gihune Jung

Jinseok Kang

Young Soo Seo

Teug Y Won

Ji Hyun Kwon

Provided is a method of manufacturing a plastic substrate with lower auto-fluorescence and better specificity. The method includes: (a) preparing a plastic substrate having an Atomic Force Microscopic (AFM) surface roughness of R&#x3c;3 nm or R&#x3c;4 nm under the condition of 50 &#x3bc;m&#xd7;50 &#x3bc;m or less; (b) treating the plastic substrate with plasma; and (c) treating the plastic substrate with a surface-modifying monomer. A plastic substrate manufactured by the method is also provided. The plastic substrate exhibits a remarkably low auto-fluorescence and thus better specificity for detection of target biomolecules, which enables the broad application of a plastic substrate, which can be easily designed to include a microfluidic structure relative to a glass substrate but has been limitedly used due to high auto-fluorescence, to microarrays, biochips, or well plates. Furthermore, the method of manufacturing the plastic substrate enables an enhancement in the surface specificity of the plastic substrate and easy adjustment of the surface characteristics of the plastic substrate.

Method of manufacturing plastic substrate using plasma process and plastic substrate manufactured using the method

An extracellular leucine aminopeptidase was isolated from the culture medium of Streptomyces therrnonitrificans which is a Zn.sup.2+ -dependent metalloenzyme which comprises an amino acid sequence Lys-Phe-Ser-Lys-Lys-Phe-Asn-Glu (SEQ ID NO: 3) at the N-terminal thereof, and amino acid sequences of Glu-Pro-Gly-Thr-Gly-Ala-Leu-Glu-Pro (SEQ ID NO: 4) and Asn-Pro-Asp-Ile-Val-Tyr (SEQ ID NO: 5) at other regions thereof. The aminopeptidase has a pH optimum of 7.5 to 9.0, a temperature optimum of 30.degree. to 50.degree. C., and an apparent molecular weight as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis under reducing conditions of 41 to 45 kDa, and as determined by gel filtration under native state conditions of 36 to 40 kDa.

Extracellular aminopeptidase isolated from Streptococcus

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Daejeon, South Korea

Young Mee Lee