National Research Council of Canada

Young-Jun Choi

Carlos B Miguez

Bernard Massie

Alaka Mullick

Methylotrophic or methanotrophic bacteria such asare transformed with a gene of interest, and expression of the gene is regulated by means of a cumate repressor protein and an operator sequence which is operatively linked to the gene of interest, and the addition of an external agent. Specifically, the cymR repressor and cmt operator frommay serve to regulate gene expression in methylotrophic or methanotrophic bacteria with the addition of cumate.

Regulation of heterologous recombinant protein expression in methylotrophic and methanotrophic bacteria

The integration of genes into methylobacterium, such asATCC is disclosed, using a transposon system, preferably the mini-Tn7 transposon system, and under the control of a promoter, such as the strong methanol dehydrogenase promoter (P). Multicopy integration of genes of interest is also disclosed. The unique and specific attachment site for the Tn7 attachment (attTn7) is identified for

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Pierrefonds, Canada

Young-Jun Choi