University of Missouri

Lekarska Fakulta V Plzni, Univerzita Karlova (faculty of Medicine in Pilsen, Charles University)

Chonbuk National University, Division of Biotechnology, College of Environmental and Bioresources

Ceska Zemedelska Univerzita V Praze

Institute of Animal Science

Young-Joo Yi

Peter Sutovsky

Milena Kralickova

Katerina Zamostna-Adamkova

Jan Nevoral

Jaroslav Petr

Media, kits, and methods for cultivation of mammalian embryos from fertilized oocyte stage to blastocyst stage in the presence of N-benzyl-3,5-dicarbethoxy-4-phenyl-1,4-dihydropyridine. The use of this supplement improves the early embryonic development, in particular the quality of zygotes and the blastocyst formation. The supplemented media can be used with pigs, humans, or other mammals N-benzyl-3,5-dicarbethoxy-4-phenyl-1,4-dihydropyridine may be added to known media, and used for with vitro fertilization methods.

Supplement for cultivation of mammalian embryos

The present invention provides a new and improved sperm stimulating additive comprising a certain amount of inorganic pyrophosphate (PPi). Addition of PPi in the media for human/animal in vitro fertilization (IVF) improves fertilization rate; addition of PPi in the semen extender for farm animal artificial insemination (AI) may improve pregnancy rates; furthermore, mammalian oocytes matured in vitro in a medium including PPi attain improved fertilization and developmental potential, while embryos cultured in medium supplemented with PPi have improved development to blastocyst.

Inorganic pyrophosphate and uses thereof

Growing community of inventors

Columbia, MO, United States of America

Young-Joo Yi