Korea Research Institute of Bioscience and Biotechnology

Dankook University Cheonan Campus Industry Academic Cooperation Foundation

Young Joo Jang

Han Oh Park

Dong Uk Kim

Mi Sun Won

Dong Sup Kim

Kyung Sook Chung

Shin Jung Choi

Hyang Sook Yoo

Min Ho Lee

Hye Mi Lee

Hyong Bai Kim

Kyung Jung Kang

Mi Young Nam

Sang Jo Han

Jo Young Park

Kwang Lae Hoe

Kyung Sun Heo

Seung Tae Baek

The present invention relates to a composition for the differentiation of dental pulp stem cells into odontoblasts and an IgG or IgM type monoclonal antibody that specifically binds to the surface of odontoblasts differentiated from the stem cells. According to the present invention, BMP2 and BMP4 are optimally combined to significantly increase the differentiation efficiency of dental pulp stem cells into odontoblasts, to induce the mineralization of the matrix, and to improve the differentiation ability of odontoblasts into dentin. Further, the IgG or IgM monoclonal antibody that specifically binds to the surface of odontoblasts of the present invention is used to effectively isolate and purify odontoblasts, which can be useful for tissue regeneration and differentiation.

IgG type monoclonal antibodies specifically binding to odontoblast surface

A method comprising transformingwith a deletion cassette, constructed by four-round serial PCR, block PCR or total gene synthesis, containing a homologous recombination site is provided for preparing gene-targeted heterozygous deletion. Also provided are gene-targeted hetero2ygous deletionmutants prepared by the method, and a library of gene-targeted heterozygous deletionmutants. Further, the library is useful in constructing a method and a kit for screening a drug's modes of action.

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Seoul, South Korea

Young Joo Jang