Lg Electronics Inc.

Seegene, Inc.

Lg Information & Communications, Ltd.

Other

Lg Infocomm Usa, Inc.

Young Jo Lee

Ki Jun Kim

Young Woo Yun

Soon Yil Kwon

Suk Hyon Yoon

Jong Yoon Chun

Jong Hoe An

Sung Lark Kwon

Sung Kwon Hong

Chan Ho Kyung

Young Cho Kim

Young Woo Yoon

Cheol Woo You

Han Bit Lee

Ki-Jun Kim

Cheolwoo You

Dae Young Kim

Young Jun Kim

Jong Sun Han

Hyung Uk Kwon

Young Wook Kim

Byung Kwan Yi

Mi Hyun Jang

In Taek Hwang

Young Sik Youn

Kyoung S Kim

Young Yong Park

Sung Moon Ko

Chang Woo Hong

Woo Seok Kang

Heejoung Lee

Jong Hyae Ahn

Chanho Kyung

Nam Suk Kim

Namsoo Park

Hyeon Ho Jeong

Joug Yoon Chun

The present invention relates to a method for predicting the melting temperature (T) of an oligonucleotide, in particular a primer or probe, in a PCR or hybridization assay. The method of present invention can accurately predict the Tof an oligonucleotide in various reaction environments using the equations for Tcalculation, the equation including parameter values optimized for the reaction environment in which the oligonucleotide is to be used.

Method for predicting the melting temperature of oligonucleotide

The present invention relates to detection of target nucleic acid sequences using different detection temperatures. The present invention employing different detection temperatures enables to detect a plurality of target nucleic acid sequences in conventional real-time manners even with a single type of label in a single reaction vessel. The conventional technologies detect a plurality of target nucleic acid sequences by a melting analysis after target amplification. Unlikely, the present invention does not require a melting analysis after target amplification, such that the time for analysis is greatly reduced.

Detection of target nucleic acid sequences using different detection temperatures

The present invention relates to a method and device for determining the presence or absence of a target analyte in a sample. The present invention may analyze the target analyte without false results, especially false positive results by using a fitting accuracy of a nonlinear function to a data set as a direct indicator for target analyte analysis.

Method and device for analyzing target analyte in sample

The present invention relates to a method for calibrating a data set of a target analyte in a sample, wherein a normalization coefficient for calibrating the data set is provided by using a reference value, a reference cycle and the data set, and the calibrated data set is obtained by applying the normalization coefficient to the signal values of the data set. The present method is very effective in removing the inter- and intra-instrument signal variations of data sets. Furthermore, since the present method can be configured in software, the instant method is capable of being applied universally to various analytical instruments (e.g., a real-time PCR instrument) regardless of manufacturer. Accordingly, the method by the present invention would be very useful in diagnostic data analysis.

Method for calibrating a data set of a target analyte using a normalization coefficient

The present invention relates to differentiating signals of interest for target nucleic acid sequences. The present invention permits to obtain an individual signal value (i.e., variable) contained in a total signal detected at detection temperatures by using mathematical equations. The present invention based on equation-solving approach enables to obtain the individual signal value in a systematical manner, thereby providing analysis results in much more accurate and convenient manner.

Differentiation of signals for target nucleic acid sequences

The present invention relates to the detection of a nucleotide variation on a target nucleic acid sequence using an amplification blocker and a VD-PTOCE (Variation Detection by PTO Cleavage and Extension) assay. The present invention is significantly effective in the detection of a minority mutation in an excess of wild-type DNA.

Detection of nucleotide variation on target nucleic acid sequence

The present invention relates to the evaluation of the performance of a component using a pair of dimer-forming primers. The method using the pair of dimer-forming primers according to the present invention can be used not only for evaluating the performance of components including a nucleic acid polymerase but also as an internal control in the detection of a target nucleic acid sequence.

Evaluation of performance of components using a pair of dimer-forming primers

The present invention relates to extraction of a signal for a target nucleic acid sequence from signals for two target nucleic acid sequences in a sample. The present invention can contribute to dramatic improvement in methods for detecting target nucleic acid sequences using different detection temperatures and reference values. The present invention using an amended reference value as well as an initial reference value can lead to increasing the detection accuracy in methods for detecting target nucleic acid sequences using different detection temperatures and reference values.

Signal extraction for a target nucleic acid sequence

The present invention relates to a method for providing an analytical signal for determination of the presence of a target nucleic acid sequence in a sample. The present invention can contribute to dramatic improvement in methods for detecting target nucleic acid sequences using different detection temperatures and reference values. The present invention allows detection of a target nucleic acid sequence in a more accurate, effective and reproducible manner, by removing or adjusting a signal region that may affect the detection of a target nucleic acid sequence.

Analytical signal for determination of the presence of a target nucleic acid sequence

The present invention relates to the detection of a target nucleic acid sequence from a DNA or a mixture of nucleic acids by a PCE-hCTO (PTO Cleavage and Extension using hCTO) assay on a solid phase. According to the present invention, the extended duplex is formed in a liquid phase in a target-dependent manner and then its presence is detected on a solid phase. Since hCTO is not immobilized onto a solid phase, the extended duplex is more effectively formed in a liquid phase.

Detection of target nucleic acid sequence on solid phase by PTO cleavage and extension using HCTO assay

The present invention relates to the detection of a target nucleic acid sequence by a PTOCE-E (PTO Cleavage and Extension-dependent Extension) assay. The PTOCE-E assay of the present invention can reduce the non-target signal and increase the target signal as compared with the conventional PTOCE and PCE-SH methods, thereby enabling more accurate detection of the target nucleic acid sequence.

Detection of target nucleic acid sequences by PTO cleavage and extension-dependent extension assay

The present invention relates to the detection of a target nucleic acid sequence by a PTOCE (PTO Cleavage and Extension) assay. The present invention detects a target nucleic acid sequence in which the PTO (Probing and Tagging Oligonucleotide) hybridized with the target nucleic acid sequence is cleaved to release a fragment and the fragment is hybridized with the CTO (Capturing and Templating Oligonucleotide) to form an extended duplex, followed by detecting the presence of the extended duplex. The extended duplex provides signals (generation, increase, extinguishment or decrease of signals) from labels indicating the presence of the extended duplex and has adjustable Tvalue, which are well adoptable for detection of the presence of the target nucleic acid sequence.

Detection of target nucleic acid sequences by PTO cleavage and extension assay

The present invention relates to the detection of a target nucleic acid sequence by a PCEC (PTO Cleavage and Extension-Dependent Cleavage) assay. The present invention is characterized by generating a cleavage site for a nucleolytic enzyme on the extended duplex of which the formation is dependent on the presence of a target nucleic acid sequence. The present invention detects the occurrence of the cleavage of the extended duplex, thereby determining the presence of the target nucleic acid sequence.

Detection of target nucleic acid sequence by PTO cleavage and extension-dependent cleavage

The present invention relates to a method for reducing a noise level of a data set for a target analyte in a sample. The present invention can reduce a noise level of a data set to a proper level conveniently by applying a noise-reduction ratio to the data set thereby the possibility of false positive may be reduced effectively. According to the present invention, the calibrated data set is obtained by using the noise-reduction ratio, so that the noise level of a data set is reduced without change of signal ratio between the data point in the data set.

Method for reducing noise level of data set for a target analyte

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Kunpo-shi, South Korea

Young Jo Lee