Genelabs Technologies, Inc.

Young J Suh

Thomas W Bruice

Gary P Schroth

Methods for determining the relative binding affinities of various ligands to various nucleic acid sequences are described. In a direct binding assay, the effect of adding increasing amounts of a ligand on a signal generated by two &ldquo;indicator&rdquo; oligonucleotides is observed. Also described is a competitive binding assay, in which a competitor oligonucleotide is added to an indicator duplex having a ligand bound thereto. The assays allow the rapid and convenient determination of nucleic acid binding specificities and base pair determinants of specificity of particular ligands.

Nucleic acid ligand interaction assays

Methods for determining the relative binding affinities of various ligands to various nucleic acid sequences, particularly in double stranded form, are described. In a direct binding assay, the effect of adding increasing amounts of a ligand on a signal generated by two &ldquo;indicator&rdquo; oligonucleotides is observed. Also described is a competitive binding assay, in which a competitor oligonucleotide is added to an indicator duplex having a ligand bound thereto. The assays allow the rapid and convenient determination of nucleic acid binding specificities and base pair determinants of specificity of particular ligands.

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Union City, CA, United States of America

Young J Suh