Samsung Electronics Co., Ltd.

Young-a Kim

Woong-Hee Lee

Kak Namkoong

Young-sun Lee

Hee-kyun Lim

Young-nam Kwon

Jeong-gun Lee

Jin-tae Kim

Young-Sun Lee

Myo-yong Lee

Yeon-ja Cho

Jung-joo Hwang

Shin-i Yoo

Sang-hyo Kim

Jang-Won Seo

Yoon-kyoung Cho

Jae Julien

Jae-Jeong Kim

Jin-Tae Kim

Chang-eun Yoo

Jung-im Han

Kwang-ho Cheong

Seung-yeon Yang

Joong-gun Lee

Mi-kyung Kim

Display screen or portion thereof with graphical user interface

Display screen or portion thereof with transitional graphical user interface

Provided is a method for quantifying an initial concentration of a nucleic acid from a real-time nucleic acid amplification data. Nucleic acid (DNA or RNA) extracted from organism or virus is amplified using an enzyme. Then, the initial concentration of the nucleic acid is found by calculating the characteristic amplification cycle number or the characteristic amplification time at which the fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid has half of its maximum value, or the characteristic amplification cycle number or the characteristic amplification time at which the amplification efficiency has the maximum or the minimum value, or the prior-to-amplification fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid. Accordingly, the initial concentration of the nucleic acid can be calculated without differentiation or integration.

Method for quantifying initial concentration of nucleic acid from real-time nucleic acid amplification data

A method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser are provided. According to the method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.

Method and apparatus for the rapid disruption of cells or viruses using micro magnetic beads and laser

Provided are methods of isolating and amplifying nucleic acids from and in a nucleic acid-containing sample. The nucleic acid isolation method includes contacting a nucleic acid-containing sample to a silanized solid support to capture nucleic acids to the silanized solid support and treating the nucleic acid-captured solid support with an alkaline solution of pH 9 to 14. The nucleic acid amplification method includes contacting a nucleic acid-containing sample to a silanized solid support to capture nucleic acids to the silanized solid support; treating the nucleic acid-captured solid support with an alkaline solution of pH 9 to 14; and adding a nucleic acid amplification solution to the resultant solution after the alkaline solution treatment to perform nucleic acid amplification.

Methods of isolating and amplifying nucleic acids using silanized solid support

Growing community of inventors

Suwon-si, South Korea

Young-a Kim