Gene Bridges Gmbh

European Molecular Biology Laboratory

Europaisches Laboratorium Fur Molekularbiologie (embl)

L'université Catholique De Louvain

Europaisches Laboratorium Fur Molekularoiologie (embl)

Youming Zhang

Jun Fu

Adrian Francis Stewart

Francis Stewart

Frank Buchholz

A Francis Stewart

Rolf Müller

Marcello Maresca

Bernard Hallet

Joep Pieter Paul Muyrers

Axel Steffen Erler

Xiaoying Bian

Philipp Martin Seibert

A method for performing homologous recombination between at least a first nucleic acid molecule and a second nucleic acid molecule which share at least one region of sequence homology. A method for improving the efficiency of homologous recombination.

Direct cloning

This invention is related to bacterial engineering and the heterologous expression of useful compounds. In particular, the invention relates to a heterologous host that has been engineered for expression of a gene which is capable of polyketide or non-ribosomal peptide synthesis. Methods of treating cancer are also disclosed.

Heterologous hosts

The invention provides a method for inserting a single stranded replacement nucleic acid into a target nucleic acid, the method comprising the steps of: a) generating a single stranded replacement nucleic acid from a double stranded nucleic acid, wherein the double stranded nucleic acid is adapted at one or both of its 5' ends such that preferential degradation of one strand and/or strand separation generates the single stranded replacement nucleic acid, wherein the single stranded replacement nucleic acid comprises a 5&#x2032; region that is identical to sequence on the target nucleic acid, a 3&#x2032; region that is identical to sequence on the target nucleic acid and optionally a replacement region between the 5&#x2032; and 3&#x2032; regions that is not identical to sequence on the target nucleic acid, b) exposing the target nucleic acid to the single stranded replacement nucleic acid under conditions suitable for recombination to occur between the single stranded replacement nucleic acid and the target nucleic acid, and c) selecting a target nucleic acid whose sequence has been altered by inclusion of said single stranded replacement nucleic acid. Other methods for modifying target nucleic acids are also provided.

Method of nucleic acid recombination

The invention relates to methods for cloning DNA molecules using recE/recT-mediated homologous recombination mechanism between at least two DNA molecules where one DNA molecule is a circular or linear DNA molecule and the second DNA molecule is a circular DNA molecule, and the second DNA molecule contains two regions with sequence homology to the first DNA molecule. Competent cells and vectors are also described.

DNA cloning method

The present invention is directed to methods and compositions for TnpI-mediated genetic engineering using therecombinase TnpI and the TnpI recombination substrates TRT or TRT', and variants thereof. In particular, the invention relates to TRT or TRT&#x2032; sequences, and variants thereof, vectors, cells and kits useful for TnpI-mediated genetic recombination, as well as methods for the use of TRT or TRT&#x2032; sequences for TnpI-mediated genetic engineering.

Tyrosine recombinase for genetic engineering

DNA cloning method relying on the E. coli recE/recT recombination system

The present invention is directed to methods and compositions for DNA subcloning using bacterial recombinase-mediated homologous recombination. The invention relates to methods for cloning, compositions comprising polynucleotides usefall as cloning vectors, cells comprising such polynucleotide compositions, and kits useful for cloning mediated by bacterial recombinases, such as RecE/T and Red&agr;/&bgr;.

Methods and compositions for directed cloning and subcloning using homologous recombination

Growing community of inventors

Heidelberg, Germany

Youming Zhang