Nisshin Seifun Group Inc.

Nippon Flour Mills Co., Ltd

Incorporated Administrative Agency National Agriculture and Food Research Organization

National Agriculture and Food Research Organization

Youichi Kurimoto

Keiko Tanaka

Shinjiro Imai

Kazumi Kitta

Hiroyuki Haraguchi

Megumi Sato

Shinichiro Arami

Yasuyuki Matsuoka

Junichi Mano

Satoshi Furui

Yasuhiro Seto

Toshiki Nakamura

Junichi Yonemaru

Goro Ishikawa

Tomoya Shinbata

Mika Saito

Hideyo Yasuda

Yoshikazu Ishihara

Patricia Lynn Vrinten

Provided is a method of detecting or quantifying a wheat species-specific DNA in a test sample by polymerase chain reaction. The method comprises a step of amplifying a nucleic acid molecule having a partial sequence of a nucleotide sequence identified as SEQ ID NO: 1 using a nucleic acid molecule in the test sample or a nucleic acid molecule extracted from the test sample as the template and using a primer pair capable of amplifying the partial sequence and a step of detecting or quantifying the amplified nucleic acid molecule.

Method for detecting and quantifying wheat endogenous gene

Provided is a method of detecting or quantifying a wheat species-specific DNA in a test sample by polymerase chain reaction. The method comprises a step of amplifying a nucleic acid molecule having at partial sequence of a nucleotide sequence identified as SEQ ID NO: 1 using a nucleic acid molecule in the test sample or a nucleic acid molecule extracted from the test sample as the template and using a primer pair capable of amplifying the partial sequence and a step of detecting or quantifying the amplified nucleic acid molecule.

Disclosed are: a method for detecting common wheat among from wheat varieties contained in a sample of interest such as a food raw material or a processed food specifically, with high sensitivity, and in a qualitative and/or quantitative manner; a method for discriminating between common wheat and a wheat variety other than common wheat (e.g., durum wheat) contained in a food raw material or a processed food and detecting the common wheat in a qualitative and/or quantitative manner; and a primer set, a nucleic acid probe, and a detection kit, each of which can be used in the methods employing a PCR method. Specifically disclosed are: a method for detecting the occurrence of common wheat in a sample of interest, which comprises carrying out a PCR method using a nucleic acid extracted from the sample as a template and using a primer comprising the nucleotide sequence represented by SEQ ID NO:5 and a primer comprising the nucleotide sequence represented by SEQ ID NO:6 and detecting the occurrence of a PCR amplification product; and a method for detecting the occurrence of common wheat in a sample of interest, which comprises carrying out a quantitative PCR method using a nucleic acid extracted from the sample as a template and using a primer comprising the nucleotide sequence represented by SEQ ID NO:5, a primer comprising the nucleotide sequence represented by SEQ ID NO:6 and a nucleic acid probe comprising the nucleotide sequence represented by SEQ ID NO:11 and detecting the occurrence of common wheat qualitatively and/or quantitatively.

Method for qualitative and quantitative detection of common wheat

The object of the present invention is to provide a wheat which accumulates a starch with a novel property by controlling the expression of the enzymes described in claims. The present invention provides a wheat, which does not express any of the following proteins (1)-(6): (1) Wheat Starch Synthase II-A1 Protein encoded by Wheat Starch Synthase II-A1 gene of SEQ ID NO:1, (2) Wheat Starch Synthase II-B1 Protein encoded by Wheat Starch Synthase II-B1 gene of SEQ ID NO:3, (3) Wheat Starch Synthase II-D1 Protein encoded by Wheat Starch Synthase II-D1 gene of SEQ ID NO:5, (4) Granule Bound Starch Synthase A1 Protein encoded by Granule Bound Starch Synthase A1 gene of SEQ ID NO:7, (5) Granule Bound Starch Synthase B1 Protein encoded by Granule Bound Starch Synthase B1 gene of SEQ ID NO:9, and (6) Granule Bound Starch Synthase D1 Protein encoded by Granule Bound Starch Synthase D1 gene of SEQ ID NO:11.

Wheat having new starch and method for producing it

Growing community of inventors

Atsugi, Japan

Youichi Kurimoto