Korea Institute of Science and Technology

Absology Co., Ltd

Youhee Heo

Ji Yoon Kang

Min Cheol Park

Kyoseon Hwang

Kyeong-Sik Shin

Jae-hoon Ji

Myung-Sic Chae

Disclosed is a method for body fluid-based neurodegenerative disease diagnosis through high-sensitive immunoassay of aggregated proteins by photooxidation-induced amplification. The method according to the present disclosure provides an effect of quantitatively analyzing aggregated proteins in the form of oligomers or monomers which are present in trace amounts in a body fluid and measures normal or abnormal protein aggregation by detecting the aggregated proteins in the form of oligomers or monomers with high sensitivity by reaction of antibody-conjugated enzymes selectively bound to the aggregated proteins with substrates and photooxidation-induced amplification, thereby allowing accurate diagnosis of a neurodegenerative disease.

Detection of aggregated proteins associated with neurodegenerative disease using a photooxidation-induced amplification immunoassay

Disclosed is a method of quantifying a biomarker with high sensitivity using photo-oxidation induced amplification. The method includes performing an enzyme-substrate reaction of a measurement sample including an enzyme labeled on any one selected from among an antibody, an aptamer, and a nucleic acid specifically bound to the biomarker, optically measuring one or more optical properties selected from among amounts of color formation, light emission, and fluorescence of a product during a photo-oxidation induced amplification process while the product resulting from the enzyme-substrate reaction is continuously exposed to light to thus perform the photo-oxidation induced amplification process, indexing a time-varying pattern of the measured optical properties, and quantifying a concentration of the biomarker included in the measurement sample by comparing an index extracted during the indexing with an index of a reference sample.

Method of quantifying biomarker with high sensitivity using photo-oxidation induced amplification

A method for providing information relevant to the diagnosis of a neurodegenerative disorder. The method includes (i) obtaining a biological sample containing vesicles from a subject, (ii) measuring the level of amyloid beta in the biological sample using an antibody specific for the N-terminus of amyloid beta, and (iii) comparing the measured level of amyloid beta in the biological sample with the level of amyloid beta in a previously prepared control sample.

Methods for providing information relevant to diagnosis of neurodegenerative disorder

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Seoul, South Korea

Youhee Heo