Amano Pharmaceutical Co., Ltd.

Research Development Corporation of Japan

Yoshiyuki Takasaki

Takaichi Ohya

Noriaki Tanaka

Kuniharu Nakai

Kenichi Takami

A bread quality-improving composition and a process for the production of bread using the composition. More particularly, a bread quality-improving composition which contains a maltotriose-forming enzyme or a maltotriose-forming enzyme, glucose oxidase and/or hemicellulase and a process for the production of dough and bread using the composition. The bread quality-improving composition renders possible the easy handling of dough without covering it with wheat flour and the like, the exclusion of additives for use in the control of water content, an improvement in elasticity and ductility of bread when baked, the prevention of bread solidification and other problems such as a reduction in bread volume when frozen dough is used and the elimination or reduction of emulsifying agents.

Bread quality-improving composition and bread producing process using

The present invention provides a novel pullulanase, methods of producing and isolating the pullulanase and methods of saccharifying starch using the pullulanase. The pullulanase of the invention works in a wide pH range, as large as pH 4-9. In addition, the pullulanase is heat stable and its optimum temperature is about 60.degree. C. Therefore, the pullulanase of the invention can be used in the saccharification of starch along with amylase, which is enzymatically active at pH 4-9, to increase the yield of saccharides.

Pullulanase, methods of producing pullulanase and methods of

A mannose isomerase having excellent properties for industrial use, such as high thermal stability and resistance to high substrate concentrations, can be produced by culturing a strain of Pseudomonas (sp. AM-9582), and extracting it from the cells of AM-9582. Mannose can be effectively produced from fructose of high concentrations using the enzyme.

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Miyazaki, Japan

Yoshiyuki Takasaki