Osaka University

Japan Science and Technology Agency

Yoshiyuki Arai

Takeharu Nagai

Kazushi Suzuki

Dhermendra Kumar Tiwari

Megumi Iwano

The present disclosure relates to a device including a reagent portion in which a chemiluminescent indicator and a chemiluminescent substrate for the indicator are disposed, and a base on which the reagent portion is formed. The chemiluminescent indicator and the chemiluminescent substrate are disposed independently from each other in the reagent portion in such a manner that the chemiluminescent indicator and the chemiluminescent substrate can react with each other when a sample is supplied to the reagent portion. The present disclosure also relates to a remote diagnosis system including an imaging terminal for detecting a luminescent signal generated when a reagent is supplied to the device and an information processing unit for processing luminescent signal data obtained by the imaging terminal. The imaging terminal and the information processing unit can bi-directionally communicate with each other via a network.

Device and determination system using same

Provided is a method of detecting a biological material, by which quantitative measurement can be performed easily. The method of detecting a biological material in a sample includes: mixing, with the sample, a fusion protein (C) in which a protein (A) capable of binding the biological material and a chemiluminescent protein (B) are fused together and a substrate for the chemiluminescent protein (B); and observing a luminescent signal from the sample, wherein the protein (A) and the protein (B) are linked in such a manner that resonance energy transfer can occur, the protein (A) is either a protein (A1) that can emit fluorescence in a state where the biological material is bound thereto or a protein (A2) capable of binding an autofluorescent molecule as the biological material, and the protein (B) can excite fluorescence or autofluorescence of the protein (A) with its luminescence energy.

Method of detecting biological material, and chemiluminescent indicator used therein

A biological specimen containing a chemiluminescence substance that generates chemiluminescence is observed in a living state under a microscope. The microscope includes a light source that outputs control light that changes the state of the chemiluminescence, a defining unit that defines the radiation pattern of the control light with which an observation surface of the biological specimen is irradiated, and a detector that detects the chemiluminescence from the biological specimen.

Microscope and method for observing biological specimen in living state

Provided is a fluorescent protein that has a fast photo-switching speed and a high photostability and that switches from a non-fluorescent state to a fluorescent state by being irradiated with light for fluorescence excitation and switches from a fluorescent state to a non-fluorescent state by being irradiated with light having a specific wavelength that does not cause fluorescence excitation. The present invention relates to (a) A protein having an amino acid sequence of Sequence ID No. 1. (b) A protein that has an amino acid sequence of Sequence ID No. 1 in which one to several amino acids are deleted, substituted, and/or added.

Fluorescent protein

Provided is an optical microscope capable of maintaining an objective lens in a focus state without adversely affecting a captured image even when a substance with a weak emission is used as an observation target object. The optical microscope includes an observation optical system capable of capturing, with an image pickup device (), an optical image of an observation target object () that has been obtained with an objective lens (), and sending out the optical image as an image signal; and an autofocus device that adjusts a focal position of the objective lens based on autofocus light emitted from a focusing light source () so as to focus on the observation target object, wherein the autofocus light is emitted from the focusing light source during a non-capturing period during which the image pickup device is not capturing an image of the observation target object.

Growing community of inventors

Osaka, Japan

Yoshiyuki Arai