Mect Corporation

Kanto Ishi Pharmaceutical Co., Ltd.

Rikagaku Kenkyusho

Teikoku Chemical Industry Co., Ltd.

Yoshiyasu Shitori

Masayoshi Ito

Tomoya Ogawa

Shoji Yoshimura

Mamoru Sugimoto

Masaaki Numata

Haruo Ogura

Kimio Furuhata

Shohei Shibayama

Yoshitaka Nagai

Toshiaki Osawa

Kinji Takada

Yuzi Matsuzaki

Satoshi Toyoshima

Shingo Sato

Sadanori Ogasawara

Nobuya Katagiri

Isao Suda

Kenji Abiko

Hideki Yamamoto

Shigeki Nunomura

Shuji Fujita

Tadami Shinuchi

Tetsuzo Kato

Yoichiro Tani

Toshio Horisaki

Atsushi Kawamura

Katsuhide Kariya

Takashi Hanafusa

Jiro Horiuchi

Ryuji Niwa

Youzi Matsuzaki

A hybridoma producing a monoclonal antibody specific for free N-acetylneuraminic acid or beta glycoconjugates thereof, is herein disclosed. The hybridoma can be generated by fusing (i) B cells of lymphocytes obtained by immunizing an animal with an N-acetylneuraminic acid or a beta-glycoconjugate thereof, and (ii) myeloma cells.

Monoclonal antibodies specific for free N-acetylfuraminic acid and

A hybridoma capable of producing a monoclonal antibody specific to a sialic acid containing glycolipid carrying N-acetylneuraminic acid residue having alpha 2.fwdarw.3 linkage is herein disclosed. The hybridoma can be generated by fusing (i) B cells or lymphocytes obtained by immunizing an animal with a sialic acid containing glycolipid carrying N-acetylneuraminic acid residue having alpha 2.fwdarw.3 linkage and (ii) myeloma cells. The monoclonal antibody produced by the hybridoma can be used for purifying gangliosides, for treating patients suffering from melanoma and for diagnosing sera.

Monoclonal antibody recognizing alpha 2.fwdarw.3 bonds

The present invention relates to a sodium salt of sialosyl cholesterol. The present compound has an excellent high water solubility, and therefore the compound is very useful as a medicine to be used for treating various diseases derived from lesions of peripheral or central nerves.

Sialosyl cholesterol and neuropathy remedy comprising the same

The present invention relates to ganglioside related compounds having, for example, the following formula: ##STR1## where R.sub.1 represents H or COCH.sub.3 (hereinafter abbreviated to 'AC'); R.sub.2 represents H or CH.sub.3 ; and R.sub.3 represents --OH, ##STR2## These ganglioside related compounds are useful as membrane receptors, tumor markers, cell growth controlling substances, etc., and useful for immunotherapy for cancer, etc.

Ganglioside related compounds and method of producing the same

Sialocylglycerolipids represented by the following general formula (I): ##STR1## wherein R.sup.1 represents a hydrogen atom or a group CH.sub.3 CO--; R.sup.2 represents an alkali metal, a hydrogen atom or a lower alkyl group; R.sup.3 represents a hydrogen atom or a group C.sub.n H.sub.2n+1 -- (n is an integer ranging from 1 to 30); and R.sup.4 represents a group C.sub.m H.sub.2n+1 -- (m is an integer ranging from 1 to 30) are herein disclosed. They are prepared by treating a corresponding compound in an alkaline aqueous solvent. The method makes it possible to provide these compounds in a high yield by a simple processes.

Sialocylglycerolipids and method for preparing the same

Sialosyl cholesterol, process for producing the same, and neuropathy

A repairing agent for cells and tissues is disclosed, which agent comprises, as an active ingredient, a compound of the general formula: ##STR1## wherein Z represents lithium, potassium, sodium, ammonium or an organic ammonium when n is 1, and Z represents calcium, barium or magnesium when n is 2.

Repairing agent for cells and tissues

Method for the production of asialoganglioside related compounds of the formula (I). ##STR1## wherein R.sup.1 is hydrogen or benzoyl, R.sup.2 is hydrogen or acetyl, R.sup.3 is hydrogen, acetyl, .beta.-D-galactopyranosyl or 2,3,4,6-tetra-O-acetyl-.beta.-D-galactopyranosyl, R.sup.7 and R.sup.8 are individually alkyl having 1 to 30 carbon atoms and Ac is acetyl.

Asialoganglioside related compounds

The present invention relates to sialic acid derivatives. According to the present invention, there is provided sialic acid derivatives having the general formula (I): ##STR1## wherein, R.sup.1 represents hydrogen, an acetyl group, a trityl group, ##STR2## (R.sup.5 represents hydrogen or an acetyl group and R.sup.6 represents hydrogen, sodium or a methyl group). R.sup.2 represents hydrogen or an acetyl group,

Sialic acid derivatives and process therefor

Novel sialic acid derivatives and galactose derivatives which are useful intermediate for the synthesis of gangliosides. Methods for the production of the sialic acid and galactose derivatives. Methods for the production of ganglioside GM.sub.3.

Sialic acid derivatives, galactose derivatives and method for producing

The present invention provides ganglioside-related compounds expressed by the following formula: ##STR1## (wherein R.sub.1 denotes a hydrogen atom or SiR.sub.3 R.sub.4 R.sub.5 (wherein R.sub.3 and R.sub.4 each denotes a methyl or phenyl group, and R.sub.5 denotes a tertiary butyl or dimethylphenylmethyl group), and R.sub.2 denotes a hydrogen atom, a trityl group, or ##STR2## (wherein M denotes an alkali metal atom)) and methods of producing the same. These ganglioside-related compounds are useful as markers for the early detection of cancer and in the immunotherapy for cancer.

Glycolipid containing N-glycolylneuraminic acid and method of producing

A sialic acid derivative having an active carbonyl group represented by the formula [I]: ##STR1## wherein R.sup.1 represents hydrogen or acetyl group; R.sup.2 represents hydrogen, a metal or a lower alkyl group; R.sup.3 represents hydrogen, hydroxyl group, or a residue removed hydrogen from an alcohol portion of an active ester; Ac represents acetyl group; and n is 1 to 20, respectively. This sialic acid derivative [I] can be utilized as a starting material for various complex having a sialic acid in the molecule since it has an active carbonyl group in the molecules so that it shows high reactivity.

Sialic acid derivatives having active carbonyl group

A new process for preparing halides of N-acetylneuraminic acids useful as an intermediate in preparing new N-acetylneuraminic acid derivatives having an excellent immunological activity is provided, which process comprises simultaneously acetylating and halogenating an esterified N-acetylneuraminic acid with the aid of an acetyl halide, and according to the process, the halides may be obtained in high yield of about 90%.

Process for preparing N-acetylneuraminic acid derivatives

Novel unnatural ceramide related compounds, for instance, (2S, 3S)-2-tetracosanamido-octadecane-1,3-diol are herein provided, which are useful as intermediates in preparing ceramide moieties employed in the preparation of glycolipids and gangliosides known as tumor markers or molecular markers and which are prepared by, for instance, subjecting a chiral compound represented by the following formula: ##STR1## to deacetal treatment.

Un-natural ceramide related compounds and preparation thereof

The present invention relates to a new process for preparing 3-O-(sodium(5-acetamide-3,5-dideoxy-alpha-D-glycero-D-galacto-2-noneuro pyranosyl)onate-1,2-di-O-tetradecyl-Sn-glycerol (3) consisting essentially of hydrolyzing a 3-O-(C.sub.1-5 alkyl(5-acetamide-4,7,8,9-tetra-O-acetyl-3,5-di-deoxy-alpha-D-glycero-D-ga lacto-2-noneuropyranosyl)onate)-1,2-di-O-tetradecyl-Sn-glycerol (1) in a single step, in an aqueous lower aliphatic alcohol solution of an alkali metal hydroxide.

Growing community of inventors

Tokyo, Japan

Yoshiyasu Shitori