Nakano Vinegar Co., Ltd.

Daicel Chemical Industries, Ltd.

Yoshiya Kawamura

Hirofumi Akano

Hajime Okumura

Takeshi Sato

Masahiro Fukaya

Mikio Yamada

Yoshio Okamoto

Shunichi Uchiyama

Kenji Tayama

Shigeru Maeda

Akira Ohki

Naoki Enomoto

Yasushi Ogasawara

Yasushi Takahashi

Toshimi Tamaki

Naho Kato

Masahiro Fujimori

Yoshinori Tsukamoto

Noriko Takahashi

Gentaro Okada

Koichi Sato

Tomoaki Fukushige

Etsuzo Entani

Hiroshi Kurosawa

Kazuo Nakamura

Yoshifumi Amano

Sachiko Furukawa

Atsushi Ishikawa

Akira Nanba

Hatagaki Keizo

Ryu Ohishi

Kazuo Yoshioka

Masashi Mizokami

Hiroaki Nakagawa

Hiroyuki Ohsuga

Nobuko Kubo

Hiroshi Takemura

Sumio Akita

Tetsuya Kibe

Chizu Ishida

Ko Furukawa

Hiroko Toda

Takayuki Kaga

Kazuyo Kajino

Haruko Izumo

Hiroaki Ebisuya

Kyo Shimada

Shigesada Iijima

Emiko Ito

Yasuyuki Kuwada

Tomohiko Fukuta

Keizo Hatagaki

Kazuhiro Ito

Yoshifumi Ohshima

A process for producing starch noodles, which comprises adding 45-55 parts by weight of hot water to 100 parts by weight of starch obtained from at least one member-selected from the group consisting of potato, sweet potato, tapioca, corn, wheat and a product thereof followed by being mixed to prepare large particles of dough and then extruding the dough under degassing at degrees of vacuum of not less than 650 Torr to produce a dough sheet. Starch noodles can be produced efficiently in simple procedures without separately preparing starch paste and without using special rollers. Further, starch noodles thus produced are highly transparent and less melted by boiling.

Process for producing starch noodles by extrusion

This invention relates to the structural gene for the membrane-bound aldehyde dehydrogenase derived from microorganisms belonging to the genus Acetobacter, said structural gene having a molecular size of about 3.6 Kb and having a restriction enzyme cleavage map as shown in FIG. 1; to a plasmid containing said structural gene; to an acetic acid bacterium transformed by said plasmid; and to an acetic acid fermentation process using said transformant.

Structural gene for membrane-bound aldehyde dehydrogenase

The present invention provides a rapid coulometric analysis for the quantitative determination of a sample substance with high reproducibility without any application of voltage etc. from the outside, as well as a galvanic cell and a device for use in said coulometric analysis. According to the present invention, a wide variety of components can be analyzed by selection of electroactive substance introduced into the galvanic cell. The present invention enables a simple and easy analysis for food components such as glutamic acid, ascorbic acid, etc., water quality such as COD, etc.

Coulometric analysis method and a device therefor

The present invention relates to a novel clinical examination method which comprises analyzing the alteration in the amount of a specific component in oligosaccharides of immunogloblin G. Human diseases such as liver diseases, malignant hypertension, immunogloblin A-nephropathy, pediatric disorders, etc., are examined in terms of the alteration in bisecting N-acetylglucosamine-containing oligosaccharides in immunogloblin G from collected humor. The present invention provides highly accurate information in a manner applicable to practical operation for examination of liver diseases, allergic diseases, malignant hypertension, immunogloblin A nephropathy, pediatric disorders, as well as aging-dependent variations and the therapeutic effect of interferon.

Method for clinical examination based on the structures of immunoglobin

The present invention provides a rapid coulometric analysis for the quantitative determination of a sample substance wherein the coulometric analysis has high reproducibility without any application of voltage, etc., from an outside source, as well as a galvanic cell and a device for use in said coulometric analysis. According to the present invention, a wide variety of components can be analyzed by selection of electroactive substance introduced into the galvanic cell. The present invention enables a simple and easy analysis for food components such as glutamic acid, ascorbic acid, etc., water quality such as COD, etc.

Provided is a novel polysaccharide derivative having a main structure of the following formula (1) in which a polysaccharide or its derivative has been chemically bonded to the inner and outer surfaces of the pores of a porous carrier at the reducing terminal of the polysaccharide or polysaccharide derivative: ##STR1## Also provided are a method of producing the novel polysaccharide derivative in which a silane agent is chemically bonded to a porous carrier and thereafter a polysaccharide having a degree of polymerization from 11 to 500 or its derivative is further chemically bonded to surface treated carrier at the reducing terminal of the polysaccharide or its derivative, and a method of producing the novel polysaccharide derivative in which a polysaccharide having a degree of polymerization from 11 to 500 or its derivative is chemically bonded to a silane agent at the reducing terminal of the polysaccharide or its derivative and thereafter the polysaccharide derivative is further chemically bonded to a porous carrier. Further provided is a separating agent for chromatography containing the novel polysaccharide derivative. The novel polysaccharide derivative has excellent solvent resistance and is useful as a separating agent for chiral resolution of chiral compounds by chromatography. The methods of the present invention efficiently give the novel polysaccharide derivative.

Separation of chiral compounds on polysaccharide supports

A polysaccharide substance having the following formula is provided: ##STR1## Also provided is a method of producing the polysaccharide substance in which an oligosaccharide having a degree of polymerization from 3 to 10 is chemically bonded to a silane agent at the reducing terminal of the resultant oligosaccharide, the oligosaccharide compound is then polymerized to an average degree of polymerization from 11 to 500 in the presence of an enzyme. Another method for producing the polysaccharide substance is provided in which an aldehyde group at the reducing terminal of an oligosaccharide having a degree of polymerization from 3 to 10 is oxidized with an oxidizing agent, and the saccharide chain of the oxidized product is polymerized to a degree of polymerization from 11 to 500 in the presence of an enzyme, followed by the addition of an acid. The polysaccharide substance has excellent solvent resistance and is useful as a separating agent for chromatography, especially for the separation of chiral compounds.

Polysaccharide substances, process for producing them and use of them

A BOD analyzer is prepared having a microbe sensor containing an oxygen electrode and a microbe membrane. The microbe membrane is made by immobilizing microorganisms belonging to the genus Klebsiella in the membrane. Specifically, the BOD analyzer has a flow cell equipped with a microbe sensor containing an oxygen electrode and a microbe membrane, and a liquid passage which is connected with an entrance of the flow cell and which is equipped with an outlet. The microbe membrane is made by immobilizing microorganisms belonging to Klebsiella oxytoca 12092 strain in a porous hydrophilic membrane having an average pore size of 0.65-3 .mu.m in diameter by using at least one gelating agent selected from alginic acid or salts thereof, agar, gellan gum, xathane gum, gelatine, carageenan, locust bean gum, methylcellulose, pectin, or pullulan. The BOD analyzer can be used for batch or continuous BOD analysis and enables carrying out BOD analysis in a short period of time.

Klebsella oxytoca ferm BP-I/3616 and immobilization thereof with a

Disclosed are a novel fructosylamine deglycase characterized by the specificity to amadori compounds of catalyzing oxidation of the compounds to produce an .alpha.-ketoaldehyde, an amine derivative and hydrogen peroxide; a method of producing the novel enzyme by cultivating microorganisms belonging to the genus Candida and having an ability of producing the novel enzyme; and a method of quantitative determination of amadori compounds by applying the novel enzyme to a sample containing amadori compounds to measure the amount of the hydrogen peroxide to be formed by the oxidation reaction or measure the amount of the oxygen to be consumed by the reaction to thereby obtain the amount of the amadori compounds from the measured value. The invention provides a novel enzyme characterized by the high specificity to the reaction with amadori compounds, especially that having therein a saccharide moiety as bonded to the .epsilon.-amino group, and also provides a method of producing the enzyme and a method of quantitative determination of amadori compounds with the enzyme. Using the enzyme, quantitative determination of amadori compounds, which is difficult by conventional enzymatic methods, is possible with ease. In particular, in measurement of the amount of a saccharified protein in a sample from a living body, which is an important index substance in diagnosis of diabetes, the enzymatic method of the invention of measuring fructosamine in the sample is hardly influenced by other interfering substances or impurities in the sample, the influence by them having been inevitable in conventional chemical methods. Accurate determination of the amount is possible by the method, and the method is free from the inconvenience of staining the kits and instruments used. For measuring glycohemoglobin, the method displays another characteristic feature that its operation is more simple and needs less labor and time than conventional methods.

Fructosylamine deglycase and a method of producing it

The present invention provides a method for measuring the amount of free sulfurous acid and bound sulfurous acid in a sample using a bacterium belonging to Thiobacillus thiooxidans or Thiobacillus ferrooxidans of in which the sample is treated with acid and/or alkali to give free sulfurous acid alone in the sample. Then, the total amount of free sulfurous acid in the sample is measured by an oxidation reaction of free sulfurous acid to sulfuric acid using a bacterium belonging to Thiobacillus thiooxidans or Thiobacillus ferrooxidans.

Apparatus for measuring free and total sulfurous acid and method of

A variety of plant diseases and damage by certain pests in agricultural and horticultural plants can be efficiently controlled without substantial chemical injuries by applying a plant-protecting composition which comprises (a) 1 part by weight of a chitosan hydrolyzate having an average molecular weight of 10,000 to 50,000, obtained by acid hydrolysis or enzymatic hydrolysis of chitosan and (b) 0.25 to 4 parts by weight of acetic acid. Any possible chemical injury to plants caused by applying the composition can be further reduced when the composition is admixed with a deproteinized juice of alfalfa leaves.

Methods for protecting vegetables, turfgrass, rice and fruit trees from

The present invention relates to a BOD analyzer comprising a microbe sensor containing an oxygen electrode and a microbe membrane. The microbe membrane is made by immobilizing microorganisms belonging to the genus Klebsiella in membrane. Specifically, the present invention relates to a BOD analyzer comprising flow cell equipped with a microbe sensor containing an oxygen electrode and a microbe membrane; and a liquid passage which is connected with the entrance of the flow cell and which is equipped with an outlet. The microbe membrane is made by immobilizing microorganisms belonging to Klebsiella oxytoca 12092 strain in a porous hydrophilic membrane having an average pore size of 0.65-3 .mu.m in diameter by using at least one gelating agent selected from the group consisting of alginic acid or salts thereof, agar, gellan gum, xanthane gum, gelatine, carageenan, locust bean gum, methylcellulose, pectin, and pullulan. The present invention also relates to improved methods of analyzing BOD by using the BOD analyzer.

Biochemical oxygen demand analyzer and methods of analysis using

There is provided a structural gene of membrane-bound alcohol dehydrogenase complex having a molecular size of about 7.0 kilo base which is derived from a microorganism belonging to the genus Acetobacter represented by Acetobacter altoacetigenes and shown by the nucleotide sequence of SEQ ID. NO. 1 and SEQ ID NO. 2. This enzyme increases the efficiency of acetic acid fermentation and may be effectively utilized for quantitative determination of alcohol.

Structural gene of membrane-bound alcohol dehydrogenase complex, plasmid

An electrophoretic medium best suited as a separation medium is composed of a gel solution and contains a water-soluble polymer and permits great improvement of the enzymic protein recovery character. A gel holder is provided with stopper members and thus can hold even a soft or slippery while maintaining a fixed shape thereof, thus permitting reliable separation and purification of an intended substance without possibility of denaturing of the substance. A slab type electrophoretic apparatus permits automatic detection of the electrophoretic state of a sample and automatic completion of the electrophoresis under optimum conditions without setting measurement condition, permits continuous measurement of the buffer solution temperature, which is important for obtaining an electrophoretic condition for maintaining the enzymic function, and permits separation and purification of a large quantity of an enzyme or like physiologically active substance in a very simple operation. An electrophoretic gel cutter cuts even large size electrophoretic gels vary accurately and safely to desired sizes and particularly cuts a soft and readily deformable electrophoretic gel of polyacrylamide or the like in a pressurized state, thus permitting accurate and safe cutting of the polyacrylamide gel, which has been very difficult to cut, without causing damage to the gel.

Electrophoretic medium for electrophoretic separation, gel holder for

An enzyme sensor, which comprises an enzyme-modified electrode and a counter electrode, wherein the enzyme-modified electrode comprises, as electrode components, an enzyme and/or an enzyme-containing substance and mediator. The enzyme sensor is useful in analysis, such as the analysis of compounds in foods or components in the living body, the diagnosis of diseases and the control of reaction processes. The preparation of the enzyme-modified electrode is also described.

Enzyme sensor

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Kounan, Japan

Yoshiya Kawamura