Director-general of Agency of Industrial Science and Technology

Agency of Industrial Science and Technology

Ministry of International Trade & Industry

Agency of Industrial Science and Technology Ministry of International

Yoshitsugu Kosugi

Ikuo Matsui

Kazuhiko Ishikawa

Hideo Suzuki

Hiroyasu Ishida

Eriko Matsui

Katsuhiko Higuchi

Satoko Kawasaki

Akira Kamibayashi

Hideoki Tanaka

Masaru Shiraki

Akio Sato

Yasutaka Tahara

Joh Yong-Goe

The invention relates to a thermophilic enzyme having &#x3b2;-glycosidase activity which comprises the amino acid sequence of SEQ ID NO: 2 in which one or a plurality of amino acid residues may be deleted, replaced or added.

Methods for making and using a thermophilic enzyme as a &#x3b2;-glycosidase

A thermostable phosphatidylethanolamine N-methyltransferase and a process for producing the enzyme are provided. A host cell is transformed with an expression vector containing DNA encoding a thermostable enzyme derived from hyper-thermophilic archaea such as Pyrococcus, the enzyme having phosphatidylethanolamine N-methyltransferase activity and an optimum temperature of 90&deg; C. or higher, and the transformed host cell is cultured.

Thermostable enzyme having phosphatidylethanolamine N-methyltransferase activity

The present invention relates to a thermostable Flap endonuclease whose optimum temperature is 75&deg; C. or more and DNA coding for (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2 or (b) a protein with Flap endonuclease activity, consisting of an amino acid sequence where in the amino acid sequence (a), one or more amino acids are deleted, substituted or added. According to the present invention, there is provided a thermostable Flap endonuclease whose optimum temperature for reaction is 75&deg; C. or more. Further, this enzyme is thermally stable, so it becomes possible to develop new techniques of conducting artificial homologous recombination or genetic shuffling highly efficiently by coupling the enzyme reaction with PCR.

Thermostable flap endonuclease derived from hyperthermophile bacterium belonging to the genus pyrococcus

Thermostable flap endonuclease derived from a hyperthermophile bacterium belonging to the genus pyrococcus

An acyl peptide hydrolase having an optimum temperature range of 90-95.degree. C. and a gene encoding the same are disclosed. With the above enzyme, it becomes possible to conduct amino terminal analysis of acylated proteins and peptides at high temperatures.

Thermostable acyl peptide hydrolase and gene encoding the same

Disclosed are a thermophilic phospholipase having an optimum temperature range of 95 to 105.degree. C. which is useful in high-temperature degumming processes in oil refining process and high-temperature processing of phospholipids; and a method for producing a thermophilic phospholipase comprising culturing a microorganism capable of producing the phospholipase in a culture medium and collecting the phospholipase from the resultant culture.

Thermophilic phospholipases and method for production thereof

The reaction of a lipase upon a fatty acid is effected by an improved method which comprises bringing the lipase bonded at multiple points to an anion-exchange residue or a carrier and a carrier having a free anion-exchange group admixed therewith into contact with a reaction mixture containing an oily substance including the fatty acid and a water-soluble substance thereby forming an oily product and a water-soluble product in the reaction mixture and subsequently separating the two reaction products.

Method for reaction of lipase upon fatty acid

A water-soluble substrate and an oily substrate are continuously reacted with immobilized lipase in a reaction vessel having vertically maintained apart upper and lower conically-shaped regions, respectively, for separation of a water-soluble product and an oily product, a plurality of lipase reaction zones each containing immobilized lipase capable of being fluidized and an agitating means, and a plurality of intermediate separation zones for separation of an oily substance and a water-soluble substance. The lipase reaction zones and the intermediate separation zones are disposed alternately between the upper and lower conically-shaped separation regions. Boundaries between the lipase reaction zones and intermediate separation zones are pervious to liquid but impervious to the immobilized lipase. The water-soluble substrate and oily substrate are passed in counterflow contact through the lipase reaction zones and intermediate separation zones and mutually contact the immobilized lipase which has been fluidized. An oily product is recovered from the upper conically-shaped separation region and a water-soluble product is recovered from the lower conically-shaped separation region.

Method for continuous reaction with fluidized immobilized lipase

A lipid hydrolyzing enzyme-containing substance is brought into contact with an insoluble carrier having a hydrophobic organic compound attached thereto in the form of a hydrophobic residue so that the lipid hydrolyzing enzyme is adsorbed by the hydrophobic residue attached to the insoluble carrier. Then, the lipid hydrolyzing enzyme adsorbed on the carrier is isolated by elution.

Growing community of inventors

Ibaraki, Japan

Yoshitsugu Kosugi