Juridical Foundation the Chemo-Sero-Therapeutic Research Institute

Teijin Limited

Yoshitaka Tajima

Hiroshi Mizokami

Yoshinobu Miyatsu

Toshinobu Nouchi

Kenichi Masuda

Hiroshi Yonemura

Keishin Sugawara

Kazuyuki Nakashima

Satoshi Adachi

Shinichi Furukawa

Yoshitaka Ushio

Masahiro Sakaguchi

Kazunari Yagi

Multimers of human serum albumin are removed from a solution of human serum albumin containing the multimers by bringing the human serum albumin solution into contact with an anion-exchanger equilibrated with a buffer containing a salt in a concentration ranging from 10 to 150 mM and having a pH value ranging from 5 to 9.5.

Method for removing multimers of human serum albumin

A method for preparing human serum albumin comprises the step of heat-treating a human serum albumin solution containing contaminants at a pH value in the proximity to the isoelectric point of the contaminants.

Method for preparing human serum albumin containing heat-treatment

The present invention provides a t-PA medicinal preparation obtained by remarkably improving the solubility and stability of t-PA or modified t-PA. Nicotinamide or a derivative thereof is incorporated into t-PA or modified t-PA-containing solvent system. If necessary, a citrate buffer solution is also used. The t-PA-containing medicinal composition is in the form of, for example, a freeze-dried preparation.

Tissue plasminogen activator medicinal composition

A plasmid for expression of human coagulation factor VIII H-chain, a plasmid for expression of human coagulation factor VIII L-chain, an animal cell transformed with either said H-chain expression plasmid or said L-chain expression plasmid or with both thereof, and a process for preparing a human coagulation factor VIII protein complex which comprises forming a transformed animal cell by introducing both said H-chain expression plasmid and said L-chain expression plasmid into said animal cell, culturing said cell to produce the human coagulation factor VIII protein complex in the culture medium and collecting the same. The process of the present invention allows for the production of a safe factor VIII at a high expression level applicable for the production of Factor VIII on an industrial scale.

Process for preparing human coagulation factor VIII protein complex

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Kumamoto, Japan

Yoshitaka Tajima