Toyo Boseki Kabushiki Kaisha

Yoshitaka Nakagiri

Shinichi Teshima

Noboru Mitsuhida

There is provided an improved quantitative analysis for the determination of a substrate or enzyme. In the analysis for the enzyme, it is reacted with a known substrate and in the analysis for the substrate, it is reacted with a known enzyme and then the resultant substance is reacted with an oxidase to produce hydrogen peroxide which is then reacted with a peroxidase, phenol derivatie and coupler to form a colored material which is then subjected to colorimetic analysis. The improvement resides in conducting the foregoing process by adding to the test sample a first reagent containing the oxidase, peroxidase and phenol derivative represented by the formula: ##STR1## wherein R.sub.1 is .circle.a a lower alkyl group having one to five carbon atoms or .circle.b the group defined in .circle.a above having a hydroxyl or sulfonic acid group, R.sub.2 is a hydrogen or halogen atom, a lower alkyl group having one to five carbon atoms, a lower acyl group having one to five carbon atoms, a lower alkylether group having one to five carbon atoms, or a lower alkoxycarbonyl group having one to five carbon atoms, said groups being unsubstituted or substituted with a hydroxyl or sulfonic acid group, and n is 0 to 4. Then, there is added to the above mixture a second reagent containing the coupler and the enzyme.

Growing community of inventors

Tsuruga, Japan

Yoshitaka Nakagiri