Nitto Boseki Co., Ltd.

Shinshu University

Yoshiro Sato

Ryo Kojima

Takeshi Nagasawa

Katsuhiro Katayama

Natsuki Sato

Toshihide Miura

Kenta Noda

Kaduho Ashizawa

Chie Satokawa

Akiko Takekawa

Kiyotaka Fujita

Disclosed is a test instrument for measuring an analyte in a liquid sample by a noble metal colloid aggregation measurement method. The test instrument involves a reaction chamber in which at least the liquid sample is to be reacted with a reagent, wherein the reagent is adhered on at least a part of a surface constituting the reaction chamber in a dried state, and the reagent enables the measurement of the analyte by a noble metal colloid aggregation measurement method. The test instrument additionally involves a supply section for supplying the liquid sample and a flow path for delivering the liquid sample that has been supplied to the supply section to the reaction chamber, wherein the liquid sample that has been supplied to the supply section is delivered to the reaction chamber through the flow path to cause the liquid sample to be brought into contact with the reagent that has been adhered in a dried state, thereby producing a difference in pressure between the supply section and the reaction chamber for the purpose of dispersing the reagent in the liquid sample. When the test instrument is used, the measurement based on an absorbance at a visible region can be achieved, the analyte can be measured accurately within a short time, and the measurement suitable for a POCT field can be achieved.

Test instrument for measuring analyte in sample by an aggregation assay using a metal colloid and using a reagent attached in a dry state in a reaction chamber, and method for measuring analyte using same

In the case of assaying the level of complex AB in a sample which likely contains the complex AB composed of substance A with another substance B, the complex is assayed by the competitive homogeneous assay method with the use of reagents including a reagent containing partner C specifically binding to substance A, a reagent containing partner D specifically binding to substance B, a reagent containing fine particles carrying substance A or an analog thereof and substance B or an analog thereof, and a reagent containing partner C specifically binding to substance A and partner D specifically binding to substance B. Thus, the complex in the sample can be easily assayed. The above method is applicable to general-purpose biochemical automatic analyzers.

Method of assaying complex and kit to be used therefor

In measuring phosphoric acid by the use of an enzyme cycling system using a dehydrogenase together with a thio-NADP, a thio-NAD, a reduced thio-NADP or a reduced thio-NAD as a coenzyme, phosphoric acid is measurable in a wide concentration range from a low concentration to a high concentration by measuring phosphoric acid after previous removal of free phosphoric acid in reagent components for the measurement.

Method for measuring phosphoric acid

By using an antibody which is covalently bonded to a water soluble polymer as an antibody to be used in a competitive immunoagglutination assay in a homogeneous system, a protein antigen at a high concentration can be accurately assayed in an undiluted system without resorting to dilution.

Method of assaying antigen and kit to be used therein

A two reagent-type kit for assaying GPT by acting GPT on L-alanine and &agr;-ketoglutarate in the presence of pyridoxal phosphate, converting the resulting pyruvate into lactate with L-lactate dehydrogenase (LDH) in the presence of reduced nicotinamide adenine dinucleotide (NADH) and measuring GPT based on a decrement of NADH, the GPT assay kit comprising a first reagent and a second reagent, one of which contains pyridoxal phosphate, L-alanine, &agr;-ketoglutarate, LDH and NADH and the other reagent contains no pyridoxal phosphate but contains L-alanine. The GPT assay kit is stable over a long period of time.

Reagent for GPT assay

There is provided an enzymatic method of rapid quantitative assay for 1,5-anhydroglucitol which is also applicable to an automated analysis device. The enzymatic method of quantitative assay for 1,5-anhydroglucitol is characterized by using a pyranose oxidase derived from,No. 52 as a pyranose oxidase. According to the assaying method of the present invention, assay for 1,5-anhydroglucitol in a specimen can be performed using an automated analysis device, results in rapid assay and improved accuracy.

Method of quantitative assay for 1,5-anhydroglucitol

A method of measuring the total ketone body in a sample comprising converting acetoacetic acid in the sample to 3-hydroxybutyric acid in advance and subsequently converting the whole 3-hydroxybutyric acid including 3-hydroxybutyric acid existing in the sample originally to acetoacetic acid to lead the reduction of nicotinamide adenine dinucleotide according to the action of 3-hydroxybutyrate dehydrogenase and a sample reagent thereof is disclosed. According to this invention an assay of the total ketone body in samples can be carried out conveniently, highly precisely and quickly.

Method of measuring the total ketone body and a sample reagent

There is provided a method of quantitative assay for 1,5-anhydroglucitol in a specimen characterized by effectively removing maltose present in the specimen using .alpha.-glucosidase. Maltose present in a specimen is previously converted into glucose by the action of .alpha.-glucosidase derived from a microorganism belonging to the genus Bacillus to remove the affect by maltose. Then 1,5-anhydroglucitol in the specimen is quantitatively determined.

A quantitative assay for 1,5-anhydroglucitol in a specimen is described. This assay comprises treating a specimen with a phosphorylating enzyme in the presence of 5 to 500 mM adenosine-5'-triphosphate in a pH range of 7.2 to 8.5, to selectively eliminate sugars other than 1,5-anhydroglucitol, and reacting the product of this reaction, without removing the residual ATP, with pyranose oxidase in the above pH range. This assay is quite rapid and suitable for performance using an automated device.

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Koriyama, Japan

Yoshiro Sato