Ajinomoto Co., Ltd.

Fujitsu Corporation

Yoshio Kawahara

Tsuyoshi Nakamatsu

Shinichi Sugimoto

Eiichiro Kimura

Osamu Kurahashi

Hisashi Yasueda

Yasuhiko Yoshihara

Yumi Matsuzaki

Nobuharu Tsujimoto

Tsuyoshi Osumi

Kazuhiko Matsui

Seiko Hirano

Gen Nonaka

Naoki Akiyoshi

Kanae Nakamura

Kiyoshi Miwa

Yoshimi Kikuchi

Hisashi Kawasaki

Yoko Asakura

Makoto Tsuchiya

Kazuhiko Totsuka

Yasutsugu Yamada

Manabu Yokomori

Shinya Goto

Sigeho Ikeda

Chizu Abe

Hisao Ito

Yoshihiro Usuda

Shigeru Nakamori

Hidetsugu Nakazawa

Harufumi Miwa

Kazuo Nakanishi

Yoshiya Gunji

Kenji Soda

Shigeho Ikeda

Yuta Nakai

Hiroki Kawashima

Toshimasa Ishii

Yosuke Koyama

Akinori Uehara

Nobuyoshi Esaki

Yukiko Ono

Sumio Inoue

Inao Oyama

Takeshi Niwa

Keiji Ishii

Eiko Shimizu

Tatsuya Yoshioka

In a method for producing a target substance utilizing a microorganism comprising culturing the microorganism in a medium to produce and cause accumulation of the target substance in the medium and collecting the target substance, there is used, as the microorganism, a mutant strain or a genetic recombinant strain constructed from a parent strain of the microorganism having a respiratory chain pathway of high energy efficiency and a respiratory chain pathway of low energy efficiency as respiratory chain pathways, and having either one or both of the following characteristics:

Method for producing substance utilizing microorganism

A plasmid isolatable from, which comprises a gene coding for a Rep protein having the amino acid sequence shown in SEQ ID NO: 8 or an amino acid sequence having homology of 90% or more to the amino acid sequence shown in SEQ ID NO: 8, and has a size of about 4.4 kb or about 6 kb, or a derivative thereof.

Plasmid autonomously replicable in coryneform bacteria

In a mobile telephone including a cameraand a microphoneloaded in front, and a cameraon the back, the sensitivity of the microphoneis set at a normal level when the camerais selected, and the sensitivity of the microphoneis enhanced when the camerais selected so that the conventional problems can be solved. To attain this, an electronic apparatus such as a mobile telephone, a PHS, a PDA, etc. capable of capturing a moving picture and a still image is loaded with a camera and a microphone capable of easily and clearly collecting voice from all directions when a user who is taking a picture captures an image in front of the user, the user himself or herself, or a target in a different direction.

Electronic apparatus equipped with image capturing device and microphone

Anmutant strain deficient in dihydrodipicolinate synthase or dihydrodipicolinate reductase is transformed with a chromosomal gene library ofand a transformant strain which can grow on a minimal medium is selected. Recombinant DNA which codes for dihydrodipicolinate synthase or dihydrodipicolinate reductase (named dapB) is obtained from the transformant.

[object Object]

A methane-utilizing microorganism capable of producing L-amino acid, for example, bacteria belonging to type I, type X or type II in the taxonomic categorization methane-utilizing bacteria such asandis cultivated in a culture medium in contact with gas containing methane which is the main source of carbon, to allow the L-amino acid to be produced and accumulated in the medium, and the L-amino acid is collected from the medium.

Methods for producing L-amino acids

A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from, preferably an enzyme that functions at a higher temperature compared with that of. PCR is performed by using the primers and chromosomal DNA ofas a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of

Genes for heat resistant enzymes of amino acid biosynthetic pathway derived from thermophilic coryneform bacteria

A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived frompreferably an enzyme that functions at a higher temperature compared with that ofPCR is performed by using the primers and chromosomal DNA ofas a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of

Thermophilic amino acid biosynthesis system enzyme gene of thermotolerant coryneform bacterium

A plasmid which is able to be isolated fromand which comprises a gene coding for a Rep protein having the amino acid sequence shown in SEQ ID NO: 4 or an amino acid sequence having homology of 90% or more to the amino acid sequence shown in SEQ ID NO: 4. and has a size of about 4.4 kb or about 6 kb, or a derivative thereof.

Anmutant strain deficient in dihydrodipicolinate synthase or dihydrodipicolinate reductase is transformed by using a chromosome gene library ofa transformant strain which can grow on a minimal medium is selected, and recombinant DNA containing DNA which codes for dihydrodipicolinate synthase or dihydrodipicolinate reductase is obtained from the transformant.

Gene encoding dihydrodipicolinate synthase from Bacillus methanolicus and methods of making lysine wing said gene

The present invention provides dihydropicolinate synthase and dihydrodipicolinate reductase enzymes from polynucleotides encoding the enzymes, and methods of producing L-lysinse in microorganisms expressing the enzymes.

Dihydrodipicolinate synthase from Bacillus methanolicus

A microorganism is utilized to fermentatively produce useful substances such as amino acids by cultivating the microorganism in a medium to allow a fermentative product to be produced and accumulated in the medium, and collecting the fermentative product, wherein the microorganism to be used is modified by introduction of at least one of a gene coding for a heat shock protein and a gene coding for a &sgr; factor which specifically functions for the heat shock protein gene to enhance expression amount of the heat shock protein in cells, whereby the microorganism is allowed to have added resistance to stress which would otherwise restrain growth of the microorganism and/or production of the fermentative product.

Stress-resistant microorganism and method of producing fermentative product

A novel insertion sequence, which has been found in the sMMO gene coding for methane monooxygenase of methane-assimilating bacterium,NCIMB 11132 strain, and has inverted repeat sequences consisting of a sequence of the nucleotide numbers 5-19 of SEQ ID NO: 1 at the both ends, can be utilized as effective means for genetic analysis including creation of insertion mutant strains, gene mapping, promoter searching, insertion of genetic information into chromosomal DNA, disruption of specific gene and the like, or utilized for improving methane-assimilating bacteria by chromosomal genetic engineering techniques.

Insertion sequence

Stress-resistant microorganism and method of producing fermentative

.alpha.-Ketoglutarate dehydrogenase gene

Growing community of inventors

Kawasaki, Japan

Yoshio Kawahara