Hamamatsu-photonics K.k.

Kyoto University

Yoshinori Mizuguchi

Norikazu Sugiyama

Shigeru Uchiyama

Masahiko Hirano

Hirotoshi Terada

Toyohiko Yamauchi

Hidenao Yamada

Norio Nakatsuji

Hideshi Oishi

Tadashi Fukami

Kiyoshi Kamiya

Yoshio Isobe

Yumi Kakuno

Hideshi Ohishi

Norifumi Tomita

Hideji Fujiwake

Iwayo Nakagawa

The present invention relates to a cell assessment method characterized in including an acquisition step of acquiring an optical path length image of a small cell clump, an extraction step of extracting a cell nucleus region within the acquired optical path length image, a comparison step of comparing an optical path length of an inside and an optical path length of an outside of the extracted cell nucleus region, and an assessment step of assessing whether or not a cell is a stem cell based on the comparison results.

Cell assessment method, cell assessment device, and cell assessment program

In a wavelength-variable light outputting apparatus, a diffraction gratingand a shielding memberwhich make wavelength and light quantity variable are attached to galvanometric scannersrespectively, and the latter are swung, whereby the wavelength can be made variable at a high speed while in a state where the light quantity is kept constant. Such an apparatus is useful for capturing a fluorescent image of a biological sample in particular. By way of the shielding memberlight is made incident on the optical fiberand is outputted therefrom, whereby a biological sample SM can effectively be irradiated with light.

Variable-wavelength optical output device

A triple view imaging apparatus is provided for measuring quantitative distribution of material or property in a sample. In the triple view imaging apparatus, an optical system receives an original optical image of the sample, separates the original optical image into at least two secondary optical images having different optical properties from one another, and projects the at least two secondary optical images into a single view angle. A single video camera simultaneously picks up the thus projected plurality of secondary optical images as a single composite image and produces image signals representing the light intensities of the plurality of secondary optical images. An image processor receives the image signals and processes the image signals to obtain final image signals representing a relationship between the image signals for respective ones of the plurality of secondary optical images. An image display receives the calculated final image signals and displays a tertiary optical image based on the calculated final image signals which defines quantitative distribution of material or property in the sample.

Triple view imaging apparatus

The present invention relates to an equipment for analyzing the Ca.sup.2+ concentration in the cardiomyocytes, wherein a fluorescent reagent is loaded into the cells to cause intracellular carboxyl groups to be coupled with Ca.sup.2+. The cells are subjected to pulse-excited lights of differing in wavelengths during a vertical blanking period following a predetermined period of time after application of the stimulative signal. The signal causes the cells to generate fluorescent images varying in fluorescence intensity due to the difference in the wavelength of the pulse-excited lights. The resultant images generated are captured by a high-sensitivity television camera and displayed on a video monitor. A plural number of image data are obtained by varying the timing of the stimulating signal with the images stored in image memory. Image data is accessed through a central processing unit which two-dimensionally analyzes the change in the shape of a single cardiomyocyte due to the contraction and the dynamic change in the intracellular Ca.sup.2+ concentration.

Image processing equipment with light source synchronized to blanking

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Hamamatsu, Japan

Yoshinori Mizuguchi