Hamamatsu-photonics K.k.

Yoshinori Hashimoto

Masafumi Oshiro

Yuichi Toyoda

An image acquisition apparatusA includes a sample stagefor placing a sample S thereon, an image pickup devicecapable of acquiring a two-dimensional image and TDI driving, an image pickup optical systemincluding an objective lens, a scanning unit for acquiring an observation image while scanning the sample S by the image pickup deviceand the image pickup optical system, and a focus oscillation control devicefor controlling oscillation of a focus in the sample S so that an image pickup focus in the sample S oscillates at an oscillation frequency fs in an optical axis direction. The focus oscillation control devicesets the oscillation frequency fs so that the oscillation frequency fs substantially coincides with Ns times (Ns is an integer not less than 1) the basic frequency, which is a reciprocal 1/Ti of an image pickup period Ti of TDI driving in the image pickup device. Thus, an image acquisition apparatus capable of efficiently acquiring an observation image with focus extended can be realized.

Image-acquisition device

An image acquiring apparatus for acquiring images of a sample includes a macro image acquiring unitfor acquiring a macro image of the sample, a dark field light sourceto be used for acquiring a dark field macro image of the sample as a macro image, a macro image processing unitwhich generates a reference macro image by processing image data of the macro image, and an image pickup condition setting unitwhich sets an image acquiring range corresponding to a range including an object of image acquisition as an image pickup condition of a micro image of the sample by referring to the reference macro image. This realizes an image acquiring apparatus, an image acquiring method, and an image acquiring program by which a macro image of a sample as an object of image acquisition is preferably acquired.

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Hamamatsu, Japan

Yoshinori Hashimoto