Hitachi, Ltd.

Yoshinori Harada

Hideki Kambara

Kenji Yasuda

Kazuo Takeda

Kenichi Kawabata

Masao Kamahori

Shin-ichiro Umemura

Keiichi Nagai

Kenichi Watanabe

Kenko Uchida

Norio Shimizu

Chusuke Munakata

Kazuaki Sasaki

Tamotu Simada

Jiro Tokita

Hiromichi Shimizu

Jirou Tokita

Mitsuru Tamura

Yuji Sasaki

Simada Tamotu

An ultrasonic manipulation apparatus has a plurality of ultrasonic wave oscillators arranged in two dimensions to trap, fix or move particles to an optional position in the solution or perform cell fusion by using a gradient force obtained by superposing one over another the gradient force fields generated by ultrasonic waves produced by a plurality of ultrasonic wave oscillators. The ultrasonic wave oscillators, functioning independently of one another, can emit ultrasonic waves with optional intensities and phases, and by using an external force produced by superposed gradient force fields generated by ultrasonic waves, particles are handled easily.

Particle handling apparatus for handling particles in fluid by acoustic radiation pressure

Using a mixed sample of a plurality of phosphoric acid compounds different in chemical structure as a sample for measurement, a .sup.31 P nuclear magnetic resonance signal obtained through polarization transfer between .sup.17 O and .sup.31 P is observed and signals obtained without polarization transfer are eliminated, whereby .sup.31 P nuclear magnetic resonance signal spectrum of an .sup.17 O-labeled phosphoric acid is selectively observed. For this purpose, a double resonance NMR detector capable of exciting .sup.17 O and .sup.31 P at the same time is connected to an NMR spectrometer, and using a pulse train which permits observation of a .sup.31 P nuclear magnetic resonance signal obtained through polarization transfer between .sup.17 O and .sup.31 P, a .sup.31 P nuclear magnetic resonance signal from the .sup.17 O-labeled phosphoric acid is observed, and signals for .sup.17 O-unlabeled .sup.31 P are eliminated.

170-Labeled phosphoric acid compound and method and apparatus for

A hydrogen bond labeling and base sequence determination method for DNA or RNA takes advantage of the complementary nature of the fundamental structure of DNA or RNA bases to bind corresponding chemical species with the base species of a denatured DNA or RNA chain. After providing an aqueous solution containing a nucleic acid, heating the aqueous solution to cleave the nucleic acid; cooling the aqueous solution and adding a base-specific labeling molecule having available hydrogen bonds to the aqueous solution, the base-specific labeling molecules bond to the available bases in a one-to-one fashion via the available hydrogen bonds. The resulting labeled molecule can be microscopically observed to determine the base sequence of the nucleic acid.

Hydrogen bond labeling and base sequence determination methods for DNA

A process for producing a protein-oriented membrane which is enhanced physically and chemically by orienting protein and cross linking the oriented protein together, is described. The proteinaceous membrane which is subjected to orientation treatment alone is weak physically and chemically, and its processing and handling are therefore difficult. However, according to the present invention, the protein after the process of orientation is cross linked together to produce a protein-oriented membrane remarkably enhanced physically and chemically.

Process for preparing protein-oriented membrane

Nucleic acid fragments labelled by radio isotape migrate at speeds that increase as the molecular weights decrease in a migration path served with a potential at both ends thereof. The migration path is constituted by a pipe-shaped gel support such as a glass tube. A ring-shaped scintillator is provided at a middle portion of the migration path to circumscribe the gel support, and whereby the beta rays are detected. A window may be formed in the glass tube at a portion opposed to the scintillator, so that the beta rays are not attenuated by the glass.

Apparatus for determining base sequence of nucleic acid

An improved electrophoresis apparatus for nucleic acid fragments in which nucleic acid fragments are separated in the order of their molecular weight in an electrophoresis cell using an electrophoretic gel for determining the base sequence of a nucleic acid, the improvement comprising a nucleic acid fragment specimen supply section for supplying the nucleic acid fragments to the electrophoresis cell, disposed thereover a molecular sieving membrane having a predetermined degree of molecular permeability for separating to remove those high molecular weight ingredients in the nucleic acid fragment specimen other than the fragments intended to be analyzed electrophoretically.

Electrophoresis apparatus for nucleic acid fragments

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Saitama-ken, Japan

Yoshinori Harada