Juridical Foundation the Chemo-sero-therapeutic Research Institute

Teijin Limited

The Chemo-sero-therapeutic Research Institute

Yoshinobu Miyatsu

Hiroshi Mizokami

Yoshitaka Tajima

Toshinobu Nouchi

Kenjiro Kawatsu

Seiichi Yokoyama

Yoshiharu Takazawa

Takami Arai

Toshihiro Maeda

Kazuyuki Nakashima

Satoshi Koyanagi

Satoshi Adachi

Kenji Nagura

Toshio Murakami

Yoshitaka Ushio

Masahiro Sakaguchi

Kazunari Yagi

Shinichi Shibata

Michele Therese Rita Lecocq

Tetsuro Tanabe

Tetsurou Tanabe

Masamichi Motoki

Pascal Charles Louis Gerkens

Tatsuya Kasugaya

A method of producing a virus in cell culture comprising at least the steps of a) providing a population of cells cultured in a cell culture medium, b) infecting the population of cells by i. inoculating the population with the virus, and ii. incubating the inoculated population so as to allow the virus to replicate and propagate, c) collecting the produced virus, thereby providing a viral harvest, and d) purifying the virus, wherein a power density of at least 15 W/m, at least 30 W/m, at least 60 W/m, at least 100 W/m, at least 120 W/mis applied to the cell culture at least during step b).

Method of viral purification

A method for purifying a modified major mite allergen obtained by the genetic recombination technique and a purified modified major mite allergen obtained by said method for purification are provided. A method for purifying a modified major mite allergen obtained by the genetic recombination technique, which comprises the purification steps: (1) Washing and recovering inclusion bodies containing a modified major mite allergen obtained by the genetic recombination technique with MF membrane; (2) Dissolving said inclusion bodies followed by refolding; (3) Concentrating a solution containing the modified major mite allergen with simultaneous removal of low molecular weight components with ultrafiltration membrane; (4) Recovering the modified major mite allergen in non-adsorbed fractions with an anion exchanger; (5) Recovering the modified major mite allergen in adsorbed fractions with a hydrophobic gel; and (6) Recovering the modified major mite allergen in adsorbed fractions with an anion exchanger, and a modified major mite allergen with high purity obtained by said method for purification.

Method for purifying modified major mite allergen

The present invention herein discloses a method for purifying human serum albumin, which is characterized in that it comprises the steps of heat-treating a human serum albumin-containing solution including impurities originated from a host cell, in the presence of a divalent cation such as calcium ion, magnesium ion, nickel ion, cobalt ion, iron ion and zinc ion to thus selectively allow the impurities to undergo agglutination. The present invention also provides the highly purified human serum albumin prepared by the foregoing method.

Method for preparing human serum albumin through heat-treatment in the presence of divalent cation

Multimers of human serum albumin are removed from a solution of human serum albumin containing the multimers by bringing the human serum albumin solution into contact with an anion-exchanger equilibrated with a buffer containing a salt in a concentration ranging from 10 to 150 mM and having a pH value ranging from 5 to 9.5.

Method for removing multimers of human serum albumin

A method for preparing human serum albumin comprises the step of heat-treating a human serum albumin solution containing contaminants at a pH value in the proximity to the isoelectric point of the contaminants.

Method for preparing human serum albumin containing heat-treatment

Medium for culturing animal cells

Growing community of inventors

Kumamoto, Japan

Yoshinobu Miyatsu