Japan Tobacco Inc.

E.i. Dupont De Nemours and Company

Virus Ikagaku Kenkyusho Inc.

Corporate Juridical Person Society for Techno-innovation of Agriculture Forestry and Fisheries

Pioneer Hi-bred International, Inc.

Pioneer Hi-bred Int'l Inc.

Japan Tabacco Inc.

Yoshimitsu Takakura

Takeshi Yamamoto

Hiroshi Tsukamoto

Yukoh Hiei

Satoru Usami

Toshiki Mine

Masako Ichikawa

Yuji Ishida

Toshihiko Komari

Naomi Oka

Toshiyuki Komori

Shigeru Kuwata

Yasuhiro Inoue

Teruyuki Imayama

Hisashi Udagawa

Hitomi Kajiwara

Marc C Albertsen

Hajime Sakai

Stanley Luck

Kazuhiro Kondo

Scott V Tingey

Ichiro Oka

Kazuharu Koga

Masakazu Kashihara

Masako Tsunashima

Hideaki Saito

Fumiki Tsutsumi

Norio Kato

Tsuyoshi Inoue

Kozue Sofuku

Toru Ito

Akira Shinjo

Yoko Hamada

Nobuyuki Kobayashi

Shoichi Suzuki

Jun Ueki

Masao Arai

Yoshiki Kuraya

Takanori Takeuchi

Hiroshi Magome

Ryo Nishiguchi

Tomoaki Kubo

An embodiment of the present invention provides a tobacco plant having a low alkaloid content. In the tobacco plant, a function of a gene encoding aspartate oxidase AO2 is suppressed.

Plant body of genuswith low alkaloid content and production method thereof

In order to provide tobacco resistant to a virus, tobacco in accordance with the present invention is arranged such that: (i) a translation initiation factor eIF(iso)4E protein which is non-functional with respect to a virus is produced or (ii) expression of a translation initiation factor eIF(iso)4E gene is suppressed; and (a) a translation initiation factor eIF4E2 protein, which is non-functional with respect to a virus, is produced or (b) expression of a translation initiation factor eIF4E2 gene is suppressed.

Virus-resistant tobacco and breeding method therefor

A virus-resistant tobacco in accordance with the present invention is such that it includes a mutation in a translation initiation factor eIF(iso)4E gene, the mutation causing production of an eIF(iso)4E protein which is non-functional with respect to a virus or suppressing expression of the eIF(iso)4E gene, or a virus-resistant tobacco in accordance with the present invention is such that an expression level of the eIF(iso)4E gene is 20% or lower as compared to a wild type.

Virus-resistant tobacco and method for creating same

The present invention provides a modified biotin-binding protein comprising an amino acid sequence represented by SEQ ID NO: 2 or its modified sequence and having a biotin-binding activity and replacement selected from the group consisting of:

Modified biotin-binding protein

The present invention provides modified tamabidin 2, which is a modified biotin-binding protein comprising an amino acid sequence represented by SEQ ID NO: 2, an amino acid sequence having one or more amino acid mutations in the amino acid sequence of SEQ ID NO: 2, or an amino acid sequence having an identity of not less than 80% to the amino acid sequence of SEQ ID NO: 2 and having biotin-binding activity, wherein an asparagine residue at position 115 of SEQ ID NO: 2 is replaced with cysteine. The modified biotin-binding protein has remarkable heat resistance.

Modified tamavidin

Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP21 polypeptide.

Drought tolerant plants and related constructs and methods involving genes encoding DTP21 polypeptides

The present invention relates to a method for detecting an antibody against a small protein encoded by the intermediate stage transcript of HHV-6 (SITH-1) in a biological sample. The method of the present invention includes the following steps: 1) providing the SITH-1 protein; 2) binding the SITH-1 protein provided in step 1) to a carrier; and 3) contacting the biological sample with the SITH-1 protein-bound carrier prepared in step 2) to detect the SITH-1 protein antibody.

Method for detecting antibody against SITH-1 in biological sample

The present invention provides a method for detecting a substance in a biological sample, a carrier for using in the method, and a kit. The method of the present invention includes 1) providing a carrier on which a biotin-binding protein is bound and providing a biotinylated protein by biotinylating a protein that specifically binds to a substance to be detected; 2) binding the biotinylated protein to the carrier provided in step 1) to produce a biotinylated protein-bound carrier; 3) mixing (a) a biological sample, and (b-i) a cell homogenate extract prepared from cells of the same species as that of the host cells used for expressing, for example, the biotin-binding protein in step 1), and a biotin-binding protein, or (b-ii) a cell homogenate extract prepared from cells of the same species as that of the host cells used for expressing, for example, the biotin-binding protein in step 1) and genetically engineered to express a biotin-binding protein, and adding the mixture to the biotinylated protein-bound carrier produced in step 2); and 4) detecting the substance specifically bound to the biotinylated protein.

Method for detecting substance in biological sample

The present invention relates to a solid support having a heat-resistant biotin-binding protein attached thereto. The present invention also relates to the use of the solid support of the present invention having a heat-resistant biotin-binding protein attached thereto. The present invention further relates to technical fields such as purification, concentration, detection and/or capture of a biotin-linked substance by means of a heat-resistant biotin-binding protein. Such a biotin-binding protein used in the solid support of the present invention is heat-resistant and is therefore useful for use in assay systems involving exposure to a temperature of 70&#xb0; C. or more.

Use of heat-resistant biotin-binding protein, and solid support having the protein attached thereto

The present invention provides an extremely useful and novel &#x3b2;-galactoside-&#x3b1;2,6-sialyltransferase having an optimum reaction pH in a neutral to alkaline range, and a nucleic acid encoding the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant &#x3b2;-galactoside-&#x3b1;2,6-sialyltransferase.

&#x3b2;-galactoside-&#x3b1;2,6-sialyltransferase, a gene encoding thereof, and a method for enhancing enzyme activity

The present invention provides a method of binding a protein to a carrier in such a way that the protein is not impaired in its function but can be allowed to act more efficiently than when it is bound directly. The method of the present invention for binding a protein to a carrier comprises:

Method of binding proteins to carriers by making use of tamavidins

The present invention relates to a modified biotin-binding protein. The modified biotin-binding protein of the present invention includes an amino acid sequence represented by SEQ ID NO: 2, an amino acid sequence having one to several amino acid mutations in the sequence represented by SEQ ID NO: 2, or an amino acid sequence having 80% or more identity to the sequence represented by SEQ ID NO: 2, and having a biotin-binding activity, wherein at least one residue selected from the group consisting of:

The present invention aims to provide novel vectors for plant transformation. The vectors of the present invention are cosmid vectors having a full length of 15 kb or less characterized in that:

Cosmid vector for plant transformation and use thereof

Growing community of inventors

Iwata, Japan

Yoshimitsu Takakura