Jnc Corporation

Fukui Prefectural University

Yoshimitsu Hamano

Hiroshi Takagi

Kazuya Yamanaka

Tomohiro Yoshimura

The invention provides a promoter derived from a genome of an actinomycete,species, and can specifically induce expression of a transgene in an actinomycete,species, in and after a logarithmic growth phase, and an actinomycete host having a high secondary metabolite production ability and a high precursor supply ability in and after the logarithmic growth phase, and a method for producing useful substances in which the promoter and the actinomycete host are combined.

Method for producing useful substances by a recombinant actinomycete, streptomyces species

An enzyme capable of reducing the antibiotic activity (i.e., toxicity) of streptothricin with respect to eukaryotic cells without sacrificing antibiotic activity with respect to prokaryotic cells is provided along with a streptothricin derivative having reduced toxicity with respect to eukaryotic cells while retaining antibiotic activity with respect to prokaryotic cells, and a manufacturing method thereof. By opening the lactam ring of streptothricin, a protein having the amino acid sequence according to SEQ ID NO: 2, 4, 6, 8, 10, 12, 14 or 16 of the invention can reduce the antibiotic activity of streptothricin D with respect to eukaryotic cells without sacrificing antibiotic activity with respect to prokaryotic cells.

Growing community of inventors

Fukui, Japan

Yoshimitsu Hamano