Eisai R&d Management Co., Ltd.

Eisai Company, Limited

Mitsubishi Kasei Corporation

Yoshimi Takai

Yuichi Ono

Masakazu Takeuchi

Toshio Imai

Yoshimasa Sakamoto

Miyuki Nishimura

Yasuko Nakagawa

Tomoya Nakatani

Eri Mizuhara

Mayumi Asano

Koji Morimoto

Yoko Inoue

Yutaka Teranishi

Jun Kondo

Yasushi Matsui

Kenji Irie

Rie Matsui

Masanori Asada

Yasuko c/o Kan Research Institute Inc Nakagawa

Yuichi c/o Kan Research Institute Inc Ono

Yoshimasa c/o Kan Research Inst Inc Sakamoto

Eri c/o Kan Research Institute Inc Mizuhara

Tomoya c/o Kan Research Institute Inc Nakatani

A novel gene 65B13 expressed specifically and transiently in dopaminergic neuron precursor cells immediately after cell cycle exit was obtained by the present invention. The cellular expression of 65B13 can be used as an index to select cells that are suitable in terms of their safety, survival rate, and network formation ability, for transplant therapy of neurodegenerative diseases such as Parkinson's disease.

Gene specifically expressed in postmitotic dopaminergic neuron precursor cells

A mouse cDNA library from gene fragments encoding proteins localizing at cell-cell junctions was screened by a technique visualizing localization of a protein to identify a junction-enriched and -associated protein, JEAP. GenBank homology search was performed based on the sequence. Based on the obtained sequence, a mouse cDNA library was screened to identify JEAP-2. By using prepared antibodies against these proteins, it was revealed that these proteins express specifically at tight junctions, in particular, tight junctions in exocrine glands.

Exocrine gland tight junction-constituting protein jeap family

Methods of isolating dopaminergic neuron precursor cells

The objective of the present invention is to provide a gene encoding novel afadin DIL domain-binding protein (ADIP) and use of the novel ADIP protein. The present inventors successfully identified a novel afadin-binding protein (ADIP) using yeast two-hybrid screening in order to identify how the nectin and afadin system organize tight junctions and adherens junctions at the cell-cell junctions. The novel protein is useful for evaluating actin cytoskeleton-controlling agents.

ADIP protein and use thereof

A protein useful for clarifying the regulation mechanism of Ca-dependent exocytosis in particular, the activation and inactivation of Rab3A, and a method of screening for a material useful for regulating Ca-dependent exocytosis in particular, the activation and inactivation of Rab3A, using the above protein. By using the coimmunoprecipitation with an anti-Rab3A GEP antibody, a protein participated in the regulation of activation or inactivation of Rab3A is determined. As the protein binds to rabconnectin-3 and GDP/GTP exchange protein, it can be used for screening for a material that increases or decreases the binding.

Rabconnectin-3-binding protein

A mouse cDNA library from gene fragments encoding proteins localizing at cell-cell junctions was screened by a technique visualizing localization of a protein to to identify a junction-enriched and -associated protein, JEAP. GenBank homology search was performed based on the sequence. Based on the obtained sequence, a mouse cDNA library was screened to identify JEAP-2. By using prepared antibodies against these proteins, it was revealed that these proteins express specifically at tight junctions, in particular, tight junctions in exocrine glands.

The present invention provides a GTP binding protein containing the following amino acid sequence, with a molecular weight of about 22K dalton and having GTP binding activity which is inhibited by N-ethyl-maleimide and GTP hydrolyzing activity: Thr-Ile-Glu-Asp-Ser-Tyr, and a method for the production of a GTP binding protein, which comprises introducing a DNA fragment containing DNA that encodes the GTP binding protein into a cloning site present at the downstream to a promoter of an expression vector, then introducing the expression vector thus constructed into a host, culturing said host, thereby expressing and accumulating the GTP binding protein and then collecting thereof.

Mutant Smg p21 protein with GTP binding activity

Growing community of inventors

Kobe, Japan

Yoshimi Takai